Invitrogen™ Phospho-PPAR alpha (Ser12) Polyclonal Antibody

Descrizione

PA1-820 detects phospho-peroxisome proliferator activated receptor (PPAR) alpha S12 from mouse tissues. PA1-820 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~52 kDa protein which corresponds to phospho-PPAR alpha S12 from mouse adipose tissue extract. The PA1-820 immunogen is a synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha. This peptide (Cat. # PEP-181) is available for use in neutralization and control experiments.

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.