Thermo Scientific™ Phosphorylating Phosphoramidite, standard grade, 20-400 finish bottle

Phosphorylating phosphoramidite can be utilized to achieve phosphorylation of the 5' oligo terminus (also known as Phosphate-ON or Chemical Phosphorylation Reagent (CPR)). Chemical phosphorylation is a cost-effective substitute to enzymatic methods (via T4 kinase and ATP), allowing efficient introduction of terminal phosphate groups. Oligos with a 5'-phosphate are conducive to a number of various applications utilizing the ligation of oligos to each other, such as NGS adapters, cloning, and gene construction. This product is supplied in a 30 mL amber bottle with a 20-400 Finish neck style, which is compatible with most Expedite Systems of oligonucleotide synthesizers.

Features:
• Full chemical name: 2-[2-(4,4-Dimethoxytrityloxy)ethylsulfonyl]ethyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula: C₃₄H₄₅F₃N₂O₇PS
• Formula weight: 656.66 g/mol
• HPLC Purity: ≥95.0%