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Thermo Scientific™ Phusion™ HF and GC Buffer Packs

Codice prodotto. 10492088 Sfoglia Tutto Thermo Scientific Prodotti
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Materiale o nome chimico:
Detergent-free FF Buffer Pack
Detergent-free GC Buffer Pack
GC Buffer Pack
HF Buffer Pack
Colore:
Colorless
Green
Dimensione della confezione:
pezzi 4
6 product options available for selection
Product selection table with 6 available options. Use arrow keys to navigate and Enter or Space to select.
Codice prodotto. Materiale o nome chimico Colore unitSize
10492088 HF Buffer Pack Colorless pezzi 4
10584578 GC Buffer Pack Colorless pezzi 4
10226009 Detergent-free FF Buffer Pack Colorless pezzi 4
10333609 Detergent-free GC Buffer Pack Colorless pezzi 4
13525170 HF Buffer Pack Green pezzi 4
13535170 GC Buffer Pack Green pezzi 4
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 6 options available.
6 options
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price
Codice prodotto. 10492088 Fornitore Thermo Scientific™ N. del fornitore F518L

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Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
TRUSTED_SUSTAINABILITY

Specifica

Materiale o nome chimico HF Buffer Pack
Colore Colorless
Descrizione Phusion™ HF Buffer Pack
Da utilizzare con (applicazione) Standard PCR
Quantità 4 x 1.5 mL
Include • 5X Phusion HF Reaction Buffer (4 x 1.5 mL)
• 50 mM MgCl2 (4 x 1.5 mL)
• DMSO (0.5 mL)

Store at –20°C.

For Research Use Only. Not for use in diagnostic procedures.

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