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Thermo Scientific™ Phusion High-Fidelity PCR Kit

Codice prodotto. 10044257 Sfoglia Tutto Thermo Scientific Prodotti
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N. di reazioni:
200 Reactions
50 Reactions
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200 reazioni
50 reazioni
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10044257 50 Reactions 50 reazioni
10084207 200 Reactions 200 reazioni
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price
Codice prodotto. 10044257 Fornitore Thermo Scientific™ N. del fornitore F553S

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62.84 EUR valido fino al 2026-03-20
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Descrizione

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Thermo Scientific Phusion High-Fidelity DNA Polymerase sets a high standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

The Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons. The DNA template amount is sufficient for performing 20 control reactions in 50 μL volume or 50 control reactions in 20 μL volume.

Highlights

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • Increased product yields with minimal enzyme amounts

Applications

  • High-fidelity PCR
  • High throughput
  • Amplification of difficult (GC-rich) templates
  • Template generation for sequencing
  • Multiplex PCR
  • Long-range PCR
  • Cloning & mutagenesis
  • Microarray

Notes

  • Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
TRUSTED_SUSTAINABILITY

Specifica

Content And Storage • Phusion DNA Polymerase (2 U/μL)
• 5X Phusion GC & HF Buffer
• dNTP mix (10 mM each)
• Control template (λ DNA) and DNA size standard
• 1.3 kb & 10 kb primers (4 μM each)
• 50 mM MgCl2 solution and DMSO

Store at –20°C.
GC-Rich PCR Performance High
Polimerasi Phusion High-Fidelity DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity PCR Kit
Quantità 50 reactions
Shipping Condition Dry Ice
Da utilizzare con (applicazione) Standard PCR, High-fidelity PCR
Fedeltà (rispetto a taq) 52X
Hot Start No
N. di reazioni 50 Reactions
Sporgenza Blunt
Reaction Format Separate Components
Dimensioni (prodotto finale) 20 kb or less
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For Research Use Only. Not for use in diagnostic procedures.

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