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  7. Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Produktkod. 10402678 Handla allt< titel> Produkter
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Include:
GC Buffer
HF Buffer
N. di reazioni:
100 Reactions
500 Reactions
Förpackningsstorlek
100 reazioni
500 reazioni
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Produktkod. Include N. di reazioni unitSize
10402678 HF Buffer 500 Reactions 500 reazioni
10523288 GC Buffer 100 Reactions 100 reazioni
10094387 GC Buffer 500 Reactions 500 reazioni
10369537 HF Buffer 100 Reactions 100 reazioni
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price
Produktkod. 10402678 missing translation for 'mfr' Thermo Scientific™ missing translation for 'supplierNo' F531L

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628.60 EUR valido fino al 2026-04-10
MIGLIOR PREZZO in promozione! Usa il codice promo "27964" per avere il tuo prezzo promozionale.
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Beskrivning

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides, and optimized reaction buffer including MgCl2. Two master mix formulations are available: with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

Highlights

  • High fidelity (25X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed

Applications

  • Amplification of difficult (GC-rich) templates
  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR

Using Phusion DNA polymerases

Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

TRUSTED_SUSTAINABILITY

Specifikationer

Concentrazione 2X
Content And Storage • 2X Phusion Master Mix with HF Buffer (10 x 1.25 mL)
• 100% DMSO (2 x 500 μL)

Store at –20°C.
GC-Rich PCR Performance Medium
Polimerasi Phusion High-Fidelity DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity PCR Master Mix
Quantità 500 reactions
Shipping Condition Dry Ice
Da utilizzare con (applicazione) Standard PCR, High-fidelity PCR
Fedeltà (rispetto a taq) 52X
Hot Start No
N. di reazioni 500 Reactions
Sporgenza Blunt
Reaction Format SuperMix or Master Mix
Dimensioni (prodotto finale) 20 kb or less
Include HF Buffer
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For Research Use Only. Not for use in diagnostic procedures.

Produkttitel
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