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Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Codice prodotto. 10639209 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
200 reactions
Dimensione della confezione:
200 reazioni
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Codice prodotto. 10639209

Marca: Thermo Scientific™ F150BID

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Descrizione

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount of sample is used directly in the PCR reaction with no prior purification steps. This allows significant savings in both time and cost.

Tested sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The kit is compatible with fresh, frozen, and even formalin-fixed, paraffin-embedded (FFPE) human tissue samples. The Phusion Human Specimen Direct PCR Kit includes optimized reagents and detailed protocols for many sample materials. The modified Phusion Hot Start II High-Fidelity DNA Polymerase utilized in the kit is a highly accurate proofreading DNA polymerase, and PCR products obtained are perfectly suitable even for cloning purposes.

Features

  • No need for time-consuming and expensive DNA purification steps
  • Two simple protocols for various applications
  • Extremely short PCR protocol times
  • Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

Notes

  • The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based DNA polymerases.
TRUSTED_SUSTAINABILITY

Specifica

Content And Storage • Phusion Human Specimen DNA Polymerase, 80 μL
• 2X Phusion Human Specimen PCR Buffer, 2 x 1.25 mL
• Universal Control Primer Mix (25 μM), 40 μL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 3 x 100 μL

Store at -20°C.
GC-Rich PCR Performance High
PCR Method Direct PCR
Polimerasi Phusion Human Specimen DNA Polymerase
Reaction Speed Fast
Product Type Direct PCR Kit
Quantità 200 reactions
Shipping Condition Wet or Dry Ice
Da utilizzare con (applicazione) Hot-start PCR
Hot Start Built-In Hot Start
N. di reazioni 200 reactions
Sporgenza Blunt
Reaction Format Separate Components
Tipo di campione Human
Starting Material DNA
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For Research Use Only. Not for use in diagnostic procedures.

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