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Thermo Scientific™ Pierce™ Biotin-Fluorescein Conjugate
Purified biotin and biotinylated BSA, HRP, AP or FITC for use as controls or to amplify signal in IHC via the avidin-biotin complex (ABC).
Brand: Thermo Scientific™ 22030
405.65 EUR valido fino al 2024-12-31
Usa il codice promo "21979" per avere il tuo prezzo promozionale.
Additional Details : CAS : 2420-94-2 Peso : 2.72160kg
Description
Thermo Scientific™ Pierce Biotinylated Proteins include biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and fluorescein (B-FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques.
Biotinylated HRP and biotinylated AP are most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method. Biotinylated Fluorescein (FITC) cannot be used to polymerize and amplify the signal to the same degree as biotinylated enzymes because each fluor molecule is tagged with only one biotin molecule. However, the fluorescent variant of traditional ABC staining is possible with this unique molecule.Highlights:
Biotinylated enzymes – biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and beta-galactosidase (B-bGal) for use in avidin-biotin complex (ABC) staining
Biotinylated FITC – amplify the fluorescein signal of biotinylated primary antibodies by using avidin or streptavidin plus biotinylated fluorescein (B-FITC)
Biotinylated controls – use biotinylated bovine serum albumin (B-BSA) or any one of these molecules as positive controls in biotin quantitation methods
Specifications
| Classic Dyes, Biotin and Analogs | |
| 5 mg | |
| 494/518 | |
| Solid | |
| Biotin Conjugate (Fluorescein) |
| Pierce Biotin-Fluorescein Conjugate | |
| Store at 4°C, protected from moisture. Shipped at room temperature. | |
| DMF (Dimethylformamide) | |
| Pierce™ |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Biotin-Fluorescein
Signal Word
- Attenzione
Hazard Category
- Lesione oculare grave/irritazione oculare Categoria 2
- Corrosione/irritazione della pelle Categoria 2
- Tossicità specifica per organi bersaglio (esposizione singola) Categoria 3
Hazard Statement
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
- H335-Può irritare le vie respiratorie.
Precautionary Statement
- P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P302+P352-IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua/.
- P304+P340-IN CASO DI INALAZIONE: trasportare l'infortunato all'aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P332+P313-In caso di irritazione della pelle: consultare un medico.
- P337+P313-Se l'irritazione degli occhi persiste, consultare un medico.
Supplemental information
- MIXTURE LIST-Contiene: Biotin-Fluorescein
For Research Use Only. Not for use in diagnostic procedures.