missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ High Capacity Ni-IMAC Resin, EDTA compatible

Pierce high-capacity EDTA-compatible Ni-IMAC resin is designed for the purification of over-expressed His-tagged proteins from cell culture media.

136.00€ - 4235.00€

Specifica

Proprietà di legame Chelator Stable
Descrizione EDTA compatible Ni-IMAC resin for His-tag protein purification
Matrix 6% crosslinked agarose
Product Line Pierce™
Tipo Resin
Visualizza altre specifiche

Prodotti 4
Codice del prodotto Marca Quantità Prezzo Quantità e disponibilità  
Codice del prodotto Marca Quantità Prezzo Quantità e disponibilità  
17173259
Vedere i documenti Dettagli della promozione
Thermo Scientific™
A50584
10 mL
136.00€
10mL
Visualizzare prodotti alternativi
Risparmia fino a 
»
Spedizione stimata: 27-05-2024
per visualizzare lo stock disponibile
Aggiungi al carrello
 
17183259
Vedere i documenti Dettagli della promozione
Thermo Scientific™
A50585
50 mL
546.00€
50mL
Visualizzare prodotti alternativi
Risparmia fino a 
»
Spedizione stimata: 27-05-2024
per visualizzare lo stock disponibile
Aggiungi al carrello
 
17193259
Vedere i documenti Dettagli della promozione
Thermo Scientific™
A50586
250 mL
2370.00€
250mL
Visualizzare prodotti alternativi
Risparmia fino a 
»
Spedizione stimata: 27-05-2024
per visualizzare lo stock disponibile
Aggiungi al carrello
 
17103269
Vedere i documenti Dettagli della promozione
Thermo Scientific™
A50587
500 mL
4235.00€
500mL
Visualizzare prodotti alternativi
Risparmia fino a 
»
Spedizione stimata: 27-05-2024
per visualizzare lo stock disponibile
Aggiungi al carrello
 
Descrizione

Descrizione

Pierce high capacity Ni-IMAC resin contains a matrix that is a 6% crosslinked agarose with optimal properties for batch, gravity column, and research/pilot scale FPLC column purifications. The resin has high binding capacity (up to 80 mg of protein per mL of resin) and can tolerate the high EDTA and reducing agent concentrations used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features of the high-capacity EDTA-compatible Ni-IMAC resin include:

  • Chelator stable—use up to 20 mM EDTA and DTT with no loss in performance
  • Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
  • High binding capacity—>80 mg/mL binding capacity for His-tagged recombinant proteins
  • Reusable—can be regenerated up to 10 times using the CIP protocol

EDTA and reducing agents such as DTT and TCEP adversely affect the performance of Ni-IMAC supports by stripping off the metal. Because of its chelator stability characteristic (EDTA tolerant), Pierce high-capacity EDTA-compatible Ni-IMAC resin is especially suited for purifying expressed His-tagged proteins that are secreted into cell culture media or for purifying intracellular His-tagged proteins that need the presence of EDTA to maintain stability and function. In both cases, large quantities of samples can be purified without the Ni metal stripping off of the support, thereby providing consistent high capacity yields.

Specifica

Specifica

Chelator Stable
6% crosslinked agarose
Resin
EDTA compatible Ni-IMAC resin for His-tag protein purification
Pierce™
Bottle
Videos
SDS
Documenti

Documenti

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.