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  5. Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 8.0

Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 8.0

Codice prodotto. 15453359
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Quantità:
250 mL
500 mL
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250mL
500mL
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Product Code. 15453359

missing translation for 'mfr': Thermo Scientific Chemicals J60618.AK

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Description

CAS: 5625-37-6 | C8H18N2O6S2 | 302.36 g/mol

PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES was used in the preparation of a variety of marine and mammalian tissues for electron microscopic study. Lipid loss and artifacts are minimized when PIPES was used to buffer the glutaraldehyde fixative. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer. Alkaline phosphatase retention activity was 60% greater.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES was used in the preparation of a variety of marine and mammalian tissues for electron microscopic study. Lipid loss and artifacts are minimized when PIPES was used to buffer the glutaraldehyde fixative. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer. Alkaline phosphatase retention activity was 60% greater.

Solubility
Fully misicible in water. Soluble in water (0.05 M), giving a clear, colorless solution (the resulting solution has a pH of approximately 4.5-4.7). 10 g will dissolve in 40 mL of 0.1 N NaOH.

Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Store away from strong oxidizing agents, bases, reducing agents, heat.
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 5625-37-6
Formula molecolare C8H18N2O6S2
Molecular Weight (g/mol) 302.36
Numero MDL MFCD00006159
InChI Key IHPYMWDTONKSCO-UHFFFAOYSA-N
PubChem CID 79723
ChEBI CHEBI:44933
IUPAC Name 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
SMILES C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

Specifications

Materiale o nome chimico PIPES buffer soln.
Colore Colorless
Concentrazione 1.0 M
pH 8.0
Forma fisica Liquid
Quantità 250 mL
Informazioni di solubilità Fully misicible in water. Soluble in water (0.05 M),giving a clear,colorless solution (the resulting solution has a pH of approximately 4.5-4.7). 10 g will dissolve in 40 mL of 0.1 N NaOH.

RUO – Research Use Only

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