Life Science Buffers

Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
- (1)
- (1)
- (93)
- (5)
- (44)
- (1)
- (2)
- (4)
- (3)
- (1)
- (1)
- (1)
- (81)
- (1)
- (117)
- (1)
- (2)
- (1)
- (2)
- (1)
- (9)
- (1)
- (1)
- (20)
- (1)
- (5)
- (9)
- (3)
- (1)
- (33)
- (2)
- (8)
- (25)
- (170)
- (1)
- (22)
- (3)
- (1)
- (2)
- (14)
- (1)
- (7)
- (6)
- (53)
- (121)
- (1)
- (1)
- (5)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (89)
- (2)
- (3)
- (3)
- (6)
- (2)
- (5)
- (550)
- (5)
- (2)
- (4)
- (4)
- (1)
- (2)
- (65)
- (30)
- (13)
- (23)
- (1)
- (8)
- (1)
- (4)
- (133)
- (7)
- (1)
- (6)
- (18)
- (32)
- (2)
- (36)
- (19)
- (24)
- (2)
- (8)
- (5)
- (14)
- (16)
- (55)
- (8)
- (46)
- (4)
- (3)
- (15)
- (8)
- (4)
- (2)
- (3)
- (4)
- (5)
- (8)
- (68)
- (4)
- (3)
- (4)
- (6)
- (7)
- (11)
- (3)
- (12)
- (4)
- (4)
- (12)
- (37)
- (2)
- (3)
- (1)
- (4)
- (2)
- (1)
- (3)
- (3)
- (3)
- (6)
- (3)
- (4)
- (1)
- (5)
- (6)
- (4)
- (1)
- (1)
- (2)
- (6)
- (1)
- (1)
- (12)
- (2)
- (3)
- (2)
- (2)
- (3)
- (7)
- (1)
- (2)
- (4)
- (2)
- (2)
- (1)
- (3)
- (2)
- (3)
- (3)
- (1)
- (2)
- (1)
- (3)
- (3)
- (3)
- (4)
- (1)
- (4)
- (3)
- (2)
- (2)
- (2)
- (4)
- (2)
- (1)
- (5)
- (12)
- (22)
- (43)
- (2)
- (9)
- (5)
- (1)
- (1)
- (52)
- (7)
- (3)
- (3)
- (27)
- (15)
- (58)
- (1)
- (13)
- (3)
- (1)
- (10)
- (1)
- (49)
- (3)
- (9)
- (1)
- (1)
- (4)
- (1)
- (1)
- (1)
- (3)
- (1)
- (29)
- (5)
- (4)
- (2)
- (2)
- (29)
- (3)
- (15)
- (4)
- (1)
- (3)
- (106)
- (1)
- (154)
- (2)
- (3)
- (6)
- (9)
- (78)
- (2)
- (40)
- (2)
- (15)
- (1)
- (14)
- (3)
- (5)
- (1)
- (5)
- (21)
- (2)
Risultati della ricerca filtrata

Fisher Bioreagents PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Tamponi di uso comune
Content And Storage | Room temperature |
---|---|
Forma | Liquid |
Fisher Bioreagents Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™
Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.
Fisher Bioreagents Compresse PBS, tampone fosfato salino, Fisher BioReagents
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
Fisher Bioreagents HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™
Agente tamponante molto diffuso
Peso formulazione | 238.197 |
---|---|
Sinonimo | N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid |
Note sul grado di purezza | DNase-, RNase- and Protease-Free |
Nota nome | 1M Solution, pH 7.3 |
Colore | Non definito |
Forma fisica | Liquido |
Conservazione consigliata | RT |
DNasi | Esente da DNasi |
Identificazione | Pass Test |
Absorbance | ≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm |
Formula molecolare | C8H18N2O4S |
CAS | 7732-18-5 |
Indice di Merck | 15, 4689 |
pH | 7.2 to 7.5 |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Materiale o nome chimico | HEPES Buffer |
Grado | Biologia molecolare |
Concentrazione | 0.95 to 1.05 M |
Fisher Bioreagents PBS, Phosphate Buffered Saline, 1X Powder, pH 7.4, Fisher BioReagents
Colore | White |
---|---|
Content And Storage | Room temperature |
Forma | Powder |
Tipo di imballaggio | Poly Bottle |
SI Analytics Soluzione di cloruro di potassio, 3M

Gli ordini eseguiti prima delle 14:00 saranno spediti oggi stesso. Gli ordini eseguiti dopo le 14:00 saranno spediti domani.
Per saperne di più
Fisher Bioreagents Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Formula molecolare: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Sinonimo: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Sinonimo | Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol |
---|---|
PubChem CID | 6503 |
Formula molecolare | C4H11NO3 |
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
SMILES | C(C(CO)(CO)N)O |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Sinonimo | TE |
---|---|
Nota nome | 1X Solution, pH 8.0 |
Pericolo per la salute 2 | CAUTION! |
Colore | Colorless |
Pericolo per la salute 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
Forma fisica | Liquid |
Conservazione consigliata | RT |
Filtered Through | Filtered through a 5-micron filter. |
DNasi | Esente da DNasi |
CAS | 7732-18-5 |
pH | 7.4 to 8.1 |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Materiale o nome chimico | Tris-EDTA |
Grado | Biologia molecolare |
Corning™ Soluzione tampone salino fosfato
SureTRACE
Supporta la tracciabilità con accesso garantito ai certificati e notifiche tempestive in caso di cambiamenti.
Per saperne di più

Supporta la tracciabilità con accesso garantito ai certificati e notifiche tempestive in caso di cambiamenti.
Per saperne di più
Used alone or in combination with other agents for washing tissues and cells.
Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)
Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.
Fisher Bioreagents TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
Sinonimo | TAE |
---|---|
Note sul grado di purezza | DNase-, RNase- and Protease-Free |
Nota nome | 50X Solution |
Forma fisica | Liquid |
Concentration or Composition (by Analyte or Components) | Tris (24%), Acetic Acid (5.0%), and EDTA (<2%). |
CAS | 6850-28-8 |
Conservazione consigliata | RT |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Filtered Through | Filtered through a 0.2-micron filter. |
Grado | Elettroforesi |
DNasi | Esente da DNasi |
Fisher Bioreagents PBS, Phosphate Buffered Saline, 1X Solution, pH 7.4, Fisher BioReagents™
Content And Storage | Room temperature |
---|---|
Forma | Liquid |
Punto di fusione | 0°C |
pH | 7.3 to 7.5 |
Tipo di imballaggio | Poly Cube |
Thermo Scientific Tris-HCl, 1M Solution, pH 8.0, Molecular Biology Grade, Ultrapure
CAS: 1185-53-1 Formula molecolare: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
PubChem CID | 93573 |
---|---|
Formula molecolare | C4H12ClNO3 |
CAS | 1185-53-1 |
Molecular Weight (g/mol) | 157.594 |
SMILES | C(C(CO)(CO)N)O.Cl |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
Fisher Bioreagents HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol
Numero MDL | MFCD00006158 |
---|---|
Note sul grado di purezza | DNase-, RNase- and Protease-Free |
Pericolo per la salute 2 | WARNING! |
Colore | White |
Pericolo per la salute 3 | Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1 |
Molecular Weight (g/mol) | 238.30 |
ChEBI | CHEBI:42334 |
Conservazione consigliata | RT |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
DNasi | Esente da DNasi |
Identificazione | Pass Test |
PubChem CID | 23831 |
Intervallo percentuale saggio | ≥99 % |
Absorbance | 0.01 max. (0.1M solution) at 280nm |
Formula molecolare | C8H18N2O4S |
Percent Purity | ≥99% |
Informazioni di solubilità | Soluble in water |
CAS | 7365-45-9 |
Indice di Merck | 15, 4689 |
pH | 5.0 to 6.5 |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Materiale o nome chimico | HEPES |
Grado | Biologia molecolare |
Sinonimo | TBS |
---|---|
Note sul grado di purezza | DNase-, RNase- and Protease-Free |
Nota nome | 10X Solution |
Colore | Colorless |
Forma fisica | Liquid |
Conservazione consigliata | RT |
DNasi | Esente da DNasi |
Punti di ebollizione | 100°C |
Concentration or Composition (by Analyte or Components) | 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl. |
CAS | 7732-18-5 |
pH | 7.5 |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Materiale o nome chimico | Tris Buffered Saline |
Grado | Biologia molecolare |