Macherey-Nagel™ Porablot Nitrocellulose membrane with supporting tissue for blotting

For membrane selection and specifications, see 'Summary of porablot membrane applicability' table.

porablot NCP

The classical transfer medium for binding proteins and nucleic acids. Membrane material: 100% nitrocelluloseMembrane character: HydrophilicPore size: 0.45μmBinding capacity: 100μg/cm²Applications: Southern, Northern and Western blotting, colony and plaque transfer, dot blottingAlso available with supporting tissue, see porablot NCL.

porablot NCL

The classical transfer medium for binding proteins and nucleic acids - the supporting tissue prevents breaking, tearing, or shrinking.

• Membrane material: 100% nitrocellulose with inert supporting tissue
• Membrane character: Hydrophilic
• Pore size: 0.45μm
• Binding capacity: 100μg/cm²
• Applications: Southern, Northern and Western blotting, colony and plaque transfer, dot blottingAlso available without supporting tissue, see porablot NCP.

porablot NY amp

The amphoteric nylon membrane with supporting tissue for improved handling.

• Membrane material: High purity nylon with inert supporting tissue
• Membrane character: Amphoteric (isoelectric point at pH6.5)
• Pore size: 0.20μm
• Applications: Southern and Northern blotting, colony and plaque lifts, dot blottingAlso available with positive surface charge, see porablot NY plus.

porablot NY plus

The strongly cationic nylon membrane with supporting tissue for improved handling.

• Membrane material: High purity nylon with inert supporting tissue
• Membrane character: Cationic (in the pH range of 3 to 10)
• Pore size: 0.45μm
• Applications: Southern, Northern and Western blotting. Very well suited for electro- and vacuum blotting techniquesAlso available with positive surface charge, see porablot NY amp.

porablot PVDF

The classical transfer membrane for Western transfer, protein binding assays.

• Membrane material: Polyvinylidene difluoride (PVDF)
• Membrane character: Hydrophobic
• Pore size: 0.20μm
• Binding capacity: 50μg/cm² to 100μg/cm² (For large, globular proteins such as immunoglobulins, for smaller peptides the binding capacity is correspondingly larger)
• Applications: Western blotting, protein binding assays, protein sequencing

• Transfer membranes from a variety of polymeric materials with a broad range of pore sizes and surface chemistry
• Easy analysis and characterisation of biomolecules
• Conservation of expensive test solutions
• Possibility of multiple investigations with one blot
• Concentration of a sample with consequent higher detection sensitivity
• Convenient storage of results
• Outstanding band resolution due to uniform, carefully controlled pore structure
• Available in custom sizes – contact our Technical Support Team for further information Transfer membranes for DNA, RNA, bacterial colonies and protein blotting applications.