Thermo Scientific™ POROS™ CaptureSelect™ AAV8 Affinity Resin

Features of POROS CaptureSelect AAV8 resin include:

• One-step AAV8 purification from crude material with high purity and yield
• High specificity and capacity, which helps reduce the process volume significantly for subsequent steps and maximizes yield
• Low operating back pressure and linear pressure versus flow responses driving flexible scalability
• Superior capacity maintained at high flow rates
• Rigid polymeric bead with covalent surface chemistry for easier handling and packing, with superior physical and chemical stability enabling a more robust downstream purification process
• Non-animal derived—the ligand is a 13-kDa single-domain fragment that comprises the 3 CDRs that form the antigen-binding domain and is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

POROS CaptureSelect AAV8 affinity resin specifically binds AAV8 and enables a robust, fast, and efficient purification process with excellent purity and yield obtained in one step. The use of this affinity resin will enable significant improvement to downstream processing by reducing the purification steps and maximizing productivity, for scalability and processing consistency.

Main characteristics:

• Matrix: cross-linked poly(styrene-divinylbenzene)
• Average particle size: 50 μm
• Ligand: POROS CaptureSelect AAV8 affinity ligand
• Ligand coupling method: carbonyldiimidazole (CDI)
• Binding capacity: >1 x 10¹³ genome copies/mL of resin
• Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
• Mechanical resistance: 100 bar (1,450 psi; 10 MPa)
• Formulation buffer: 18% (v/v) ethanol