Invitrogen™ Progesterone Receptor Monoclonal Antibody (Alpha PR6)

Descrizione

MA1-411 reacts with high affinity to the B form of the progesterone receptor from human, mouse, rat, rabbit, bovine, ovine, chicken, and guinea pig tissues. This antibody does not cross-react with estrogen receptor or glucocorticoid receptor. MA1-411 has been successfully used in Western blot, immunocytochemical, immunohistochemical and immunoprecipitation procedures. By Western blot, this antibody detects a 110 kDa protein representing the B form of the PR in chickens.' The MA1-411 immunogen is progesterone receptor purified from chick oviduct cytosol. Reconstitute with PBS.

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.