PUMA alpha Polyclonal Antibody, Invitrogen™

Descrizione

The PA5-20008 immunogen is located within the first 50 amino acids of PUMA. The immunogenic sequence is identical between the A and B forms of PUMA. In Western blot applications, this antibody detects a band at ∽23kDa. A suggested positive control is K562 cell lysate. PA5-20008 can be used with blocking peptide PEP-0128. Predicted species reactivity based on immunogen sequence: Rat: (79%), Mouse: (79%)

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.