Sartorius Vivapure™ D Maxi H IEX Spin Column

Vivapure Maxi Ion Exchange (IEX) spin columns are centrifugal devices, incorporating Sartobind Membrane Adsorber technology as their chromatography matrix. Vivapure IEX spin columns make protein purification as easy as filtration. The devices are ready-to-use and do not bear the risk of running dry. For many protein purification applications, they can replace time-consuming and tedious column chromatography. The rapid 1-2-3 bind-wash-elute protocol especially lends itself to screening applications, where many different samples are processed in parallel. VIvapure Maxi is available in Diethylamine (D) membrane chemistry.

Typical Applications

• Sample preparation prior to 1-D or 2-D PAGE
• Purification scouting of an unknown protein
• Removal of endotoxins from monoclonal antibodies
• Preparation of heme moiety from heme containing protein for functional analysis
• Polishing of His-tagged proteins
• General protein purification
• Detergent removal from protein solutions
• Purification of antibodies from serum, ascites or tissue culture supernatant
• Establishing a purification/ pre-purification protocol for a given protein
• HPLC/FPLC sample preparation
• Purification of membrane-bound proteins

Estimated protein binding capacity: 60 to 80mg

• Fast centrifugal spin columns
• Easy and ready-to-use
• Ideal for parallel processing