SIGLEC11 Polyclonal Antibody, Invitrogen™

Descrizione

Despite the predicted molecular weight, Siglec11 often migrates at a higher molecular weight in SDS-PAGE, presumably due to post-translational modifications. A suggested positive control is HepG2 cell lysate. PA5-20743 can be used with blocking peptide PEP-0857.

Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to alpha2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist.