IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector

pDSG-IBA vectors for MEXi mammalian expression system

• Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
• Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
  • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
  • Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
  • Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number

Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.