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IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression
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Vettore:
pPSG-IBA 105
pPSG-IBA 123
pPSG-IBA 164
pPSG-IBA 168
pPSG-IBA 23
pPSG-IBA 25
pPSG-IBA 3
pPSG-IBA 33
pPSG-IBA 5
pPSG-IBA 62
pPSG-IBA 63
pPSG-IBA 64
pPSG-IBA 65
pPSG-IBA wt1
pPSG-IBA103
pPSG-IBA143
pPSG-IBA145
pPSG-IBA162
pPSG-IBA167
pPSG-IBA35
pPSG-IBA43
pPSG-IBA45
Dimensione della confezione:
5µg
Descrizione
pPSG-IGA E. coli expression vectors:
pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.
- High-level expression in E. coli by bacteriophage T7 promoter
- High-level transcription by T7 RNA polymerase in BL21 strains
- High-level expression of non-toxic proteins
- Induction by IPTG
- Suitable for in vitro transcription/translation
StarGate System
IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:
- Minimal extra modification of the gene due to short combinatorial sites
- Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
- GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
- Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
- The final expression vector, the Destination Vector, is then placed into the respective host
Specifica
Specifica
| Formato | Liquid |
| Da utilizzare con (apparecchiatura) | StarGate™ System, E. Coli BL21(DE3) |
| Agente inducente | E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3)) |
| pH | 8 |
| Promotore | T7 |
| Product Type | pPSG-IBA wt1 |
| Tampone di conservazione | 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg |
| Content And Storage | 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Protein Tag | Untagged |
| Concentrazione | 250 ng/μL |
| Vedi altri risultati |
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