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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression
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Descrizione
The pYSG-IBA vector allows the high-level expression of proteins in yeast.
- The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
- The expressed recombinant protein will be localized in the cytoplasm.
- High-level expression in yeast with high copy number
- Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
- Tightly regulated expression due to Cu++-inducible CUP1 promoter
- Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
- Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
- URA3 auxotrophy marker for selection after transformation
Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specifica
Specifica
| Formato | Liquid |
| Da utilizzare con (apparecchiatura) | StarGate™ System, Yeast Host Cells |
| Agente inducente | Copper sulfate |
| Gene marcatore | leu2-d and URA3 |
| pH | 8 |
| Promotore | CUP1 |
| Product Type | Acceptor Vector for yeast expression |
| Tampone di conservazione | 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg |
| Content And Storage | 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Protein Tag | Untagged |
| Vedi altri risultati |
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