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  6. IBA Lifesciences Strep-Tactin™ TACS Agarose

IBA Lifesciences Strep-Tactin™ TACS Agarose

Codice prodotto. 17267750 Sfoglia Tutto IBA Lifesciences Prodotti
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Capacità (sistema metrico):
4 mL
20 mL
50 mL
Dimensione della confezione:
20mL
4mL
50mL
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Codice prodotto. Capacità (sistema metrico) unitSize
17267750 50 mL 50mL
17247750 4 mL 4mL
17257750 20 mL 20mL
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Codice prodotto. 17267750 Fornitore IBA Lifesciences N. del fornitore 66350025

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Descrizione

50% suspension for traceless affinity cell and exosome isolation

  • Strep-Tactin™ TACS Agarose contains agarose macrobeads coated with the streptavidin variant Strep-Tactin™.
  • A special crosslinking permits the use for cell isolation and depletion approaches.
  • It was developed for column-based Traceless Affinity Cell Selection (Fab-TACS™/Nano-TACS™).
  • In this approach, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin™ on the agarose matrix.
  • After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin™ Agarose by biotin addition.
  • In this way, the eluted cells are ready for further downstream applications.
  • The same principle is applicable for exosome isolation (Fab-TACS™ for exosomes)
  • Alternatively to Fab-Streps/Nano-Streps, it is possible to bind Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding) to this resin.
  • This makes Strep-Tactin™ TACS Agarose also suitable for negative cell selection or depletion approaches.
  • The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography.
  • To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.

Specifica

Capacità di legame Biotin Binding Capacity > 40 nmol/ml resin
Capacità (sistema metrico) 50 mL
Formulazione 50% suspension in buffer, pH 8: 100 mM Tris-HCl pH 8.0, 1 mM EDTA, 150 mM NaCl, 0.01% sodium azide
Support Type Crosslinked agarose
Da utilizzare con (applicazione) For Packing of Gravity Flow Columns for Affinity Chromatographic Cell or Exosome Isolation

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