IBA Lifesciences Strep-Tactin™XT Buffer Set

Strep-Tactin™XT Buffer Set contains all buffers necessary for purification of Strep-tag™II or Twin-Strep-tag™ fusion proteins with Strep-Tactin™XT as well as for subsequent regeneration of the resin. 10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin™ and Strep-Tactin™XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag™II and Twin-Strep-tag™ fusion protein purification. 10x Buffer BXT is a 10x concentrated Strep-Tactin™XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin™XT with very high affinity but reversibly and, thereby, allows the efficient elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins from the resin. Please note that 10x Buffer BXT is not applicable for elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins from Strep-Tactin™, since biotin irreversibly binds to these resins, and, thus, Strep-Tactin™ cannot be regenerated. Buffer XT-R is a ready-to-use Strep-Tactin™XT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-Tactin™XT after the elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins. Please note that the Strep-Tactin™XT Buffer Set is not applicable for Strep-Tactin™.