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IBA Lifesciences Strep-Tactin™XT Superflow™ High Capacity Column
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- Strep-Tactin™XT Superflow™ high capacity columns contain a 6% agarose coupled with the streptavidin variant Strep-Tactin™XT and are applicable for purification of Strep-tag™II or Twin-Strep-tag™ fusion proteins via gravity flow.
- They enable the addition of denaturing agents such as urea during the purification procedure.
- In comparison to Strep-Tactin™XT Superflow™, Strep-Tactin™XT Superflow™ high capacity has a higher density of immobilized Strep-Tactin™XT, which leads to a 3- to 5-fold higher yield of purified protein.
- As with any other Strep-Tactin™XT resins, the unspecific protein binding is extremely low, which results in an outstanding purity of the target protein.
- To reduce the waiting period during the sample and buffer application, the use of the application aid WET FRED is recommended.
Specifica
Specifica
| Capacità (sistema metrico) | 5 x 0.2 mL |
| Concentrazione | 6% agarose, crosslinked |
| Formulazione | Ready-to-use column with Strep-Tactin™XT high capacity resin |
| Contenuto kit | Pre-packed in buffer, pH 8.0: 100 mm Tris-HCl pH 8.0, 1 mm EDTA, 150 mm NaCl, 0.02% sodium azide, 1.5 M a mmonium sulphate |
| Content And Storage | 2°C to 8°C |
| Quantità | 5 x 0.2 mL |
| Da utilizzare con (applicazione) | Purification of Strep-Tag™II and Twin-Strep-Tag™ Fusion Proteins |
| Tipo | Pre-packed Column |
Esclusivamente per scopi di ricerca.
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