Thermo Scientific™ pMCS minP-Tluc16-DD Vector for Luciferase Assays

The Thermo Scientific pMCS Tluc16 Vectors are multiple cloning site (MCS) vectors designed to accept a promoter sequence for study of gene regulation using the intracellular TurboLuc™16 (Tluc16) luciferase reporter.

• Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning sites provide versatility for transfer of regulatory elements from one vector to another
• Synthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-through
• Dual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay
• Optimized minimal core promoter (minP) and 5' UTR region for efficient expression of Tluc16 luciferase
• Hygromycin resistance gene for use as selection marker to generate mammalian stable cell lines
• Prepared pNF-κB, and pCRE Tluc16-DD Vectors are designed to monitor the activation of NFκB protein and cAMP-binding protein (CREB) in mammalian cells
• Prepared pEF1α variety includes a modified version of the Elongated Factor 1α (EF1α) promoter for constitutive Tluc16 luciferase expression in mammalian cells