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Descrizione
TrueStart Hot Start Taq DNA Polymerase is designed for hot start PCR, a technique shown to improve specificity, sensitivity and yield of DNA amplification during PCR. TrueStart Hot Start Taq DNA Polymerase is chemically modified by adding proprietary heat-labile blocking groups to amino acid residues. TrueStart Hot Start Taq DNA Polymerase is inactive at room temperature because of this modification, avoiding extension of non-specifically annealed primers or primer dimers.
TrueStart Hot Start Taq DNA Polymerase activates rapidly during the initial denaturation step of PCR. The lack of additional activation step differentiates it from other hot start DNA polymerases, making this enzyme an ideal tool for both automatic hot start PCR and fast PCR. Activated TrueStart Hot Start Taq DNA Polymerase catalyzes 5'-3' synthesis of DNA, lacks detectable 3'-5' exonuclease (proofreading) activity, but possesses low 5'-3' exonuclease activity. These two activities are not detectable before activation.
Highlights
Specifica
Specifica
| Descrizione | DNA polimerasi TrueStart Hot Start Taq |
| Quantità | 500 U |
| Da utilizzare con (applicazione) | PCR hot start ad alta resa; RT-PCR; amplificazione altamente specifica di stampi genomici e di cDNA fino a 3 kb; amplificazione dei target di DNA a basso numero di copie; PCR in tempo reale;, RT-PCR, Real Time PCR (qPCR) |
| Include | 2 x 1.25 ml 10X tampone TrueStart Taq e 2 x 1.25 ml 25mM MgCl2 |
In certain countries use of this product is covered by patents. Purchase of product in these countries includes non-transferable, limited license for using only this amount of product for the purchaser's own internal research.
This product or the use of this product is covered by US patent application US20070224611A1 and corresponding counterparts owned by Fermentas. The purchase of this product includes a non-transferable license to use this product for the purchaser's internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license from Fermentas.
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