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Thermo Scientific™ Kit per la sintesi di cDNA Verso™
Descrizione
Con il sistema Verso si ottiene una trascrizione inversa robusta e sensibile grazie alla combinazione di un enzima RT a elevata affinità, di un esclusivo metodo di innesco dell'RNA e di un sistema di buffering ottimizzato. L'enzima Verso RT possiede un'elevata affinità al template RNA e una minore attività di RNasi H, per trascrivere anche le sezioni con un'alta struttura secondaria. Il metodo di innesco con oligo(dT) intensifica ulteriormente la sensibilità aumentando l'efficienza della trascrizione e il tampone di sintesi del cDNA è stato ottimizzato in modo da ottenere un pool di cDNA completo e variegato.
The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool. The Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.
Features of the Verso cDNA Synthesis Kit include:
• Highly sensitive with a broad dynamic range—can reverse transcribe RNA from an input range of 1pg to 1μg
• Efficient RT reaction allows for 75% shorter protocol times (down to 30 minutes)
• Unique priming strategy for increased efficiency and broad template flexibility
• High affinity RT enzyme effectively transcribes through difficult RNA sequences
• Wide working temperature range (42–57°C) for success with GC-rich and other difficult templates
• Included RT enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment
• RNase inhibitor included
• Can be paired with any PCR Kit for complete 2-Step RT-(q)PCR
Applications
• 2-step RT-(q)PCR
• Single stranded cDNA synthesis
Specifications
Specifications
| Content And Storage | • RT Enzyme mix with RNase Inhibitor |
| Formato | Kit |
| Reaction Speed | 30 min. |
| Tecnica | Reverse Transcription |
| Optimal Reaction Temperature | 42°C |
| Quantità | 40 x 20 μl Rxns |
| Reverse Transcriptase | Verso |
| Ribonuclease H Activity | Yes |
| Shipping Condition | Wet or Dry Ice |
| Da utilizzare con (applicazione) | Real Time PCR (qPCR), RT-PCR |
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For Research Use Only. Not for use in diagnostic procedures.
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