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PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.
PCR Supplies (689)
Thermal Cycler Accessories (64)
Thermal Cyclers (56)
Oligonucleotides (24)

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1 – 15 di 473 risultati
1
Promozioni disponibili

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

EDGE
Purity or Quality Grade UP (Ultra Pure)
Polimerasi AmpliTaq Gold DNA Polymerase
Linea di prodotti TaqMan
PCR Method qPCR
Formato Bottle
Da utilizzare con (apparecchiatura) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Metodo di rilevamento Primer-probe
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Standard
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
Tipo di campione Environmental Sample, Food
Concentrazione 2X
2
Promozioni disponibili

Thermo Scientific™ Exo-Resistant Random Primer

Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.

EDGE
3
Promozioni disponibili

Thermo Scientific™ Trascrittasi inversa Maxima™ H Minus

Trascrittasi inversa altamente termostabile e ad elevata processività priva di attività di RNasi H.

EDGE
Shipping Condition Dry Ice
Tecnica Reverse Transcription
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Tipo di reagente Reverse Transcription
Tipo di prodotto finale First-Strand cDNA
Dimensioni (prodotto finale) Up to 20 kb
Formato Stand-alone Enzyme
Reaction Format Separate components
Reaction Speed 15 to 30 min.
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C to 55°C
Starting Material RNA
Concentrazione 200 U/μL
4

DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance Low
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Hot-start PCR
Polimerasi DreamTaq DNA Polymerase
Sporgenza 3'-A
Concentrazione 2X
5

Thermo Scientific™ DNA polimerasi DreamTaq

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.

EDGE
GC-Rich PCR Performance Low
Reaction Speed Standard
Hot Start No
Polimerasi DreamTaq DNA Polymerase
Sporgenza 3'-A
Concentrazione 5 U/μL
Fedeltà (rispetto a taq) 1X
6

Thermo Scientific™ DNA polimerasi Phusion™ Hot Start II High-Fidelity

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Hot Start High-Fidelity PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Hot-start PCR,High-fidelity PCR
Polimerasi Phusion Hot Start II High-Fidelity DNA Polymerase
Sporgenza Blunt
Concentrazione 2X
Dimensioni (prodotto finale) 20 kb or less
Fedeltà (rispetto a taq) 52X
7

Thermo Scientific™ Kit di sintesi di cDNA a singolo filamento RevertAid™ Premium

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

EDGE
Shipping Condition Dry Ice
Tecnica Reverse Transcription
Content And Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.
Reverse Transcriptase RevertAid
Tipo di reagente Reverse Transcription
Tipo di prodotto finale First-Strand cDNA
Dimensioni (prodotto finale) Up to 13 kb
Formato Kit
Reaction Format Separate components
Reaction Speed 60 min.
Ribonuclease H Activity Yes
Da utilizzare con (applicazione) RT-PCR
Optimal Reaction Temperature 42°C
Starting Material RNA
8
Promozioni disponibili

Thermo Scientific™ Primer Oligo(dT)18

Thermo Scientific Oligo(dT)18 Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.

EDGE
Shipping Condition Dry Ice
Forma Liquid
Primer Length 18-mer
Product Type Primer
Da utilizzare con (applicazione) cDNA Synthesis
Primer Oligo dT
Concentrazione 0.5 μg/μL
9

Thermo Scientific™ Kit per la sintesi di cDNA Verso™

Si puì eseguire una trascrizione dell'RNA robusta per creare un pool di cDNA completo.

EDGE
Shipping Condition Wet or Dry Ice
Tecnica Reverse Transcription
Reverse Transcriptase Verso
Tipo di reagente Reverse Transcription
Reaction Format Separate components
Reaction Speed 30 min.
Ribonuclease H Activity Yes
Da utilizzare con (applicazione) Real Time PCR (qPCR),RT-PCR
Optimal Reaction Temperature 42°C
Starting Material RNA
Tipo di prodotto finale First-Strand cDNA
Dimensioni (prodotto finale) Up to 11 kb
Formato Kit
10
Promozioni disponibili

Thermo Scientific™ DNA polimerasi T4

Ensure accuracy in DNA synthesis with template-dependent T4 DNA Polymerase that catalyzes the 5'-3' synthesis from primed single-stranded DNA.

EDGE
Polimerasi T4 DNA Polymerase
11

Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

EDGE
Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Cloning,Hot-start PCR
Polimerasi Phire Hot Start II DNA Polymerase
Starting Material DNA
Sporgenza Blunt
Concentrazione 2X
Fedeltà (rispetto a taq) 2X
12
Promozioni disponibili

Thermo Scientific™ dTTP

Thermo Scientific dTTP (2'-deoxythymidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

EDGE
13
Promozioni disponibili

Thermo Scientific™ dNTP Mix (25 mM each)

Thermo Scientific dNTP Mix (25 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 25 mM.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Forma Liquid
Label or Dye Unlabeled
Da utilizzare con (applicazione) Standard PCR,High-fidelity PCR,RT-PCR,Real Time PCR (qPCR)
Concentrazione 25 mM
14

Thermo Scientific™ Set dNTP

Thermo Scientific dNTP Set is a convenient set of 100 mM aqueous solutions of each of dATP, dCTP, dGTP and dTTP, supplied in separate vials.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Forma Liquid
Label or Dye Unlabeled
Da utilizzare con (applicazione) Standard PCR,High-fidelity PCR,Real Time PCR (qPCR)
Concentrazione 100 mM
15
Promozioni disponibili

Thermo Scientific™ Trascrittasi inversa Maxima

Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.

EDGE
Concentrazione 200 U/μL