Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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Thermo Scientific™ HindIII

The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.

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Specifiche

Concentrazione	10 U/µl

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Thermo Scientific™ FastDigest™ HindIII

The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Prezzi e disponibilità
Specifiche

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Hind III
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Type IIS RE	No
Optimal Reaction Temperature	37°C
Sporgenza	5' protruding ends
Inactivation Temperature	80°C
Product Line	FastDigest

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Thermo Scientific™ FastDigest™ EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Prezzi e disponibilità
Specifiche

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	EcoR I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Type IIS RE	No
Optimal Reaction Temperature	37°C
Sporgenza	5' protruding ends
Inactivation Temperature	80°C
Product Line	FastDigest

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Thermo Scientific™ FastDigest SmiI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

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Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

Prezzi e disponibilità
Specifiche

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Not I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Type IIS RE	No
Optimal Reaction Temperature	37°C
Sporgenza	5' protruding ends
Inactivation Temperature	80°C
Product Line	FastDigest

Promozioni disponibili

Thermo Scientific™ FastDigest™ NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

Prezzi e disponibilità
Specifiche

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Nco I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Type IIS RE	No
Optimal Reaction Temperature	37°C
Sporgenza	5' protruding ends
Inactivation Temperature	65°C
Product Line	FastDigest

Promozioni disponibili

Thermo Scientific™ FastDigest PvuI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

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Thermo Scientific™ EcoRI

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.

Prezzi e disponibilità
Specifiche

Concentrazione	10 U/µl

Promozioni disponibili

Thermo Scientific™ SalI

The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer.

Prezzi e disponibilità
Specifiche

Concentrazione	10 U/µl

Promozioni disponibili

Thermo Scientific™ PstI

The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).

Prezzi e disponibilità
Specifiche

Concentrazione	10 U/µl

Promozioni disponibili

Thermo Scientific™ NcoI

The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I).

Prezzi e disponibilità
Specifiche

Concentrazione	10 U/µl

Promozioni disponibili

Thermo Scientific™ FastDigest™ PstI

The FastDigest PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BspMAI).

Prezzi e disponibilità
Specifiche

Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme
Enzyme	Pst I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Type IIS RE	No
Optimal Reaction Temperature	37°C
Sporgenza	3' protruding ends
Product Line	FastDigest

Promozioni disponibili

Thermo Scientific™ KpnI

The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I).

Prezzi e disponibilità
Specifiche

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Kpn I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	Unique Buffer (10X Buffer KpnI)
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentrazione	10 U/μL

Promozioni disponibili

Thermo Scientific™ DraI

Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.

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Thermo Scientific™ XhoI

Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI).

Prezzi e disponibilità
Specifiche

Concentrazione	10 U/μL

Restriction Enzymes

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