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Hot Start PCR Reagents and Kits

In hot start PCR, key reagents are prevented from reacting in the PCR mixture until optimal temperatures are met. This reduces non-specific DNA amplification that occurs naturally at lower temperatures, increasing product yield, specificity, and sensitivity.
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1

DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance Low
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Hot-start PCR
Polimerasi DreamTaq DNA Polymerase
Sporgenza 3'-A
Concentrazione 2X
2
Promozioni disponibili

Thermo Scientific™ DNA polimerasi Phire™ Hot Start II

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.

EDGE
Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance Medium
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Cloning,Hot-start PCR
Polimerasi Phire Hot Start II DNA Polymerase
Starting Material DNA
Sporgenza Blunt
Fedeltà (rispetto a taq) 2X
3
Promozioni disponibili

Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

EDGE
Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Cloning,Hot-start PCR
Polimerasi Phire Hot Start II DNA Polymerase
Starting Material DNA
Sporgenza Blunt
Concentrazione 2X
Fedeltà (rispetto a taq) 2X
4

Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

GC-Rich PCR Performance High
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Hot-start PCR,Hot-start PCR
Concentrazione 2X
Dimensioni (prodotto finale) 5 kb or less
Fedeltà (rispetto a taq) 1X
Formato Tube
5

Invitrogen™ PCR Master Mix Starter Pack

The PCR Master Mix Starter Pack is composed of three different PCR master mixes for easy evaluation of their suitability for use in your research.

6

DreamTaq™ Hot Start DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

EDGE
GC-Rich PCR Performance Low
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Hot-start PCR
Polimerasi DreamTaq DNA Polymerase
Sporgenza 3'-A
Concentrazione 5 U/μL
7

Macherey-Nagel™ NucleoType Mouse PCR, DirectPCR kit for genotyping of mouse samples

Kit for rapid mouse typing experiments with common samples such as tail clips, ear punch, hair, and blood.

Content And Storage NucleoType HotStart PCR Master Mix (2x), Lysis Buffer M, Proteinase K. Store at 4 °C or -20 °C
Tempo di attivazione Lysis 2 min; Enzyme inactivation 3 min; PCR cycling 30–90 min
Metodo di rilevamento Primer-probe
Resa 100 - 600 ng amplicon per 10 μL PCR
Da utilizzare con (applicazione) Genotyping
Tipo di campione Mouse tail clipping (1 mm), mouse ear punch (Dia. 1 mm), mouse blood (1 μL), mouse hairs (approx. 3–30)
Durata utile 1 year
Dimensioni (prodotto finale) Amplicon size: up to 1000 bp
Formato Kit
8

Macherey-Nagel™ NucleoType Seed PCR, DirectPCR kit for genotyping of seed

Time and money saving seed genotyping workflow.

Content And Storage Lysis Buffer P; Proteinase K; NucleoType HotStart PCR Master Mix (2x) containing polymerase, dNTPs, buffer, enhancer, loading dye and stabilizer. Store at 4°C or -20°C
Reaction Speed 30 to 90 min.
Tipo di kit Simple sample preparation suitable for hot start PCR
Da utilizzare con (applicazione) Genotyping
Tipo di campione Hard plant material like e.g., seeds from soybeen, wheat, corn, rice, as well as from moss, fern leaf, and fir needle
Dimensioni (prodotto finale) Amplicon size up to 2000 bp
Formato Kit
9

Abnova™ 2 x HotTaq PCR Mix with MgCl2

HotTaq PCR mix is a prepared solution containing everything needed for successful PCR reaction except specific primers and DNA template.

10

Thermo Scientific™ Thermo-Start™ Taq DNA Polymerase PCR Kts and Master Mxes

Hot-start modified for PCR applications requiring maximum specificity and sensitivity to detect rare or complex targets and allow for room-temperature reaction setup.

11

DreamTaq™ Hot Start Green PCR Master Mix, 1000 rxns

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

12

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Product Type DNA Polymerase
Polimerasi AmpliTaq Gold DNA Polymerase
Attività esonucleasi 5' - 3'
Sporgenza 3'-A
Concentrazione 5 U/μL
Dimensioni (prodotto finale) 5 kb or less
Fedeltà (rispetto a taq) 1X
13

Thermo Scientific™ Maxima Hot Start Taq DNA Polimerasi

Enhance the specificity, sensitivity, and yield of DNA amplification with this hot start DNA polymerase.

14

DreamTaq™ Hot Start Green DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps

15

DreamTaq™ Hot Start Green DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps