Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.