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Proteintech™ AuthentiKine™ Human HGF ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous HGF
Marca: Proteintech™ KE00168-96T
Dettagli aggiuntivi : Peso : 0.15000kg
Descrizione
- Recognizes human HGF
- HGF most potent mitogen of mature hepatocytes in primary culture
- Dericed from biologically inactive single chain precursor fo 728 amino acids localized on cell surface and extracellular matrix
- HGF is pleiotropic cytokine which exerts variety of effects on several cells, being involved in regulating many biological processes
Process:
- Antibody specific for HGF pre-coated onto microwells
- HGF protein in samples captured by the coated antibody after incubation
- After extensive washing, horseradish peroxidase (HRP)-conjugated detection antibody specific for HGF is added to detect captured HGH protein
- For signal development, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifica
| BC130284 | |
| 0.26 ng/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Human | |
| Human | |
| <10% | |
| Blue ice | |
| 3.5 hr. |
| 0.313 to 20 ng/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 3082 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P14210 |