C14orf126 Polyclonal Antibody, Invitrogen™

Descrizione

Immunogen sequence: WVEVQRGLVI YVCFFKGADK ELLPKMVNTL LNVKLSETEN GKHVSILDLP GNILIIPQAT LGGRLKGRNM QYHSNSGKEE GFELYSQFVT LCEKEVAANS KCAEARVVVE HGTYGNRQVL KLDTNGPFTH LIFE Highest antigen sequence identity to the following orthologs: Mouse - 84%, Rat - 85%.

Deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids. Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr). By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS (PubMed:29410408). Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site (PubMed:29410408). Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo EEF1A1/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation. [UniProt]