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Descrizione
Perform efficient synthesis of first strand cDNA from RNA templates with the complete system in Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit.
The kit utilizes the recombinant M-MuLV Reverse Transcriptase which exhibits lower RNase H activity than AMV reverse transcriptase. The recombinant RiboLock RNase Inhibitor, supplied with the kit effectively protects RNA template from degradation. The oligo(dT)18 anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail, e.g., micro RNAs. Gene-specific primers may also be used with the kits.The first strand of cDNA can be directly used as a template in PCR (see RT-PCR protocol) or in second strand cDNA synthesis (see protocol).
- Efficient synthesis of first strand cDNA up to 9kb
- Reaction temperature 37°C
- Supplied with the recombinant RiboLock RNase Inhibitor
- Complete—oligo(dT)18 and random hexamer primers included with the kit
Recommended for:
First strand cDNA synthesis for RT-PCR and real-time RT-PCR; Construction of cDNA libraries; Generation of probes for hybridization; aRNA synthesis.
Specifica
Specifica
| Product Type | Fermentas™ First Strand cDNA Synthesis Kit |
| Sufficiente per | 100 reactions |
| Da utilizzare con (applicazione) | RT-PCR |
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