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Cytiva Colonne rProtein A FF HiTrap™
Per la purificazione preparativa di anticorpi monoclonali e policlonali da surnatanti di colture cellulari, siero e asciti. Le colonne Cytiva Life Sciences™ HiTrap™ rProtein A FF sono preimpaccate con rProtein A Sepharose™ Fast Flow.
Marca: Cytiva 17-5079-01
Dettagli aggiuntivi : Peso : 0.08910kg
Descrizione
- Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
- Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
- Average particle size: 90μm
- Designed for use with a syringe, a peristaltic pump, or a chromatography system
- Available as 1mL and 5mL columns
- Ligand: Recombinant protein A (E.coli)
- Matrix: Highly cross-linked 4% agarose
- Binding capacity: ∽50mg human IgG/mL
- pH stability working range: 3 to 10
1mL columns
- Bed dimensions: 7 x 25mm
- Flow velocity: <4mL/min.
- Bed dimensions: 16 x 25mm
- Flow velocity: <20mL/min.
Specifica
| ≈50 mg IgG umane/ml | |
| Per purificazione preparativa di anticorpi monoclonali e policlonali da supernatanti in colture cellulari, siero e asciti | |
| Da 4° a 30 °C |
| <4 ml/min. | |
| Per siringa, pompa peristaltica o sistema cromatografico | |
| 5 x 1 ml |