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Cytiva blood genomicPrep Mini Spin Kit
Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow and nucleated red blood cells.
Marca: Cytiva 28-9042-64
Dettagli aggiuntivi : CAS : 9048-46-8 Peso : 1.99580kg
Includes: Spin columns with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge
collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.
Descrizione
The purification process involves minimal shearing with typical yields of 4 to 6 μg of genomic DNA per 200μL sample with volumes accommodated up to 1mL. The method uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a novel silica-membrane. The elution volume can be modified as necessary for most downstream molecular biological applications.
- High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing
- Demonstrated application to various types of whole blood including human, horse, rabbit, rat and mouse
- Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
- Validated in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests
Specifica
| illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells. | |
| Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card | |
| illustra blood genomicPrep Mini Spin Kit | |
| 50 μl whole blood, Buffy coat, bone marrow cells, 2 μl nucleated blood†, 300 μl whole blood‡ | |
| 4-6 μg/200 μl whole blood |
| Used for several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digestsSuitable for use in molecular biology applications including standard, multiplex and quantitative PCR; cloning; haplotyping; rest | |
| 50 Preps | |
| 1.7 | |
| < 20 min |
Sicurezza e movimentazione
Avvertenza
Categoria di pericolo
Indicazioni di pericolo
Consigli di prudenza
- Pericolo
Categoria di pericolo
- Tossicità acuta Categoria 4
- Lesione oculare grave/irritazione oculare Categoria 1
- Corrosione/irritazione della pelle Categoria 2
- PERICOLO A LUNGO TERMINE PER L'AMBIENTE ACQUATICO Cronico 3
Indicazioni di pericolo
- H302-Nocivo se ingerito.
- H318-Provoca gravi lesioni oculari.
- H315-Provoca irritazione cutanea.
- H412-Nocivo per gli organismi acquatici con effetti di lunga durata.
Consigli di prudenza
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P273-Non disperdere nell'ambiente.
- P301+P312-IN CASO DI INGESTIONE accompagnata da malessere: contattare un CENTRO ANTIVELENI/medico/
- P310-Contattare immediatamente un CENTRO ANTIVELENI/un medico .
- P501-Smaltire il prodotto/recipiente in .