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Thermo Scientific™ Reagenti di marcatura di massa tandem aminoreattivi Pierce™
The Thermo Scientific TMTzero Label Reagent serves as a 'light' tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
Marca: Thermo Scientific™ 90067
Dettagli aggiuntivi : Peso : 2.72160kg
Descrizione
The amine-reactive, NHS-ester-activated compounds covalently attach to the peptide amino terminus and free amino termini of lysine residues of peptides and proteins with high efficiency, thereby labeling all peptides in each sample regardless of enzyme used for digestion. Since TMT reagents share an identical structure and mass (i.e., isotopomers), labeled peptides co-elute during LC separation and are co-isolated during MS/MS analysis, resulting in fewer missing peptide identifications among samples. During MS/MS analysis, each isobaric tag is also fragmented to produce a unique reporter ion mass that is used for sample identification and quantitation. Protein quantitation is accomplished by comparing the relative intensities of the two reporter ions in the MS/MS spectra.
Specifica
| Reagente di marcatura Pierce TMTzero, 5 x 0.8 mg | |
| TMTzero™ | |
| Tandem Mass Tag™ (TMT) Labels | |
| Protein | |
| Protein Labeling |
| Mass Spectrometer | |
| Store at –20°C. | |
| Cell Lysate | |
| Mass Spectrometry | |
| 5 x 0.8 mg |
For Research Use Only. Not for use in diagnostic procedures.