Rabbit anti-Goat IgG (H+L) Secondary Antibody, Invitrogen™

Descrizione

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31105 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31105 reacts with the heavy chains of goat IgG and with the light chains common to most goat immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Country of origin: USA NOTE: For blocking, we recommend using a 4-5% normal serum from the same host species of the labeled secondary antibody. Using BSA or dry milk to block or dilute anti-bovine IgG, anti-goat IgG, anti-horse IgG and anti-sheep IgG and/or primary antibody may significantly increase background and/or reduce secondary antibody titer since BSA and dry milk may contain IgG which reacts with these antibodies.

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.