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Thermo Scientific™ DNA polimerasi Taq, nativo

Optimize amplification of DNA samples of low purity with this highly thermostable, native DNA polymerase for routine PCR.

Marca:  Thermo Scientific™ EP0282

150.00 EUR valido fino al 2024-03-29
Usa il codice promo "21615" per avere il tuo prezzo promozionale.



Dettagli aggiuntivi : Peso : 0.00100kg

Codice prodotto. 10829230

  • 225.00€ / 500 unità
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Descrizione

Descrizione

Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.

Taq DNA Polymerase (native, with BSA) is supplied with BSA as a stabilizing agent. This version of Taq DNA Polymerase is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

Highlights

  • Thermostable -half life is more than 40 min at 95°C.
  • Generates PCR products with 3'-dA overhangs.
  • Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).
Specifica

Specifica

Contains:
• 100 μL Taq DNA Polymerase, native (5 U/μL)
• 2 x 1.25 mL 10X Taq Buffer with KCl
• 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO4
• 2 x 1.25 mL 25 mM MgCl2

Store at -20°C.
Low
Standard
Standard PCR
1 X
3'-A
DNA
Primer-probe
Taq DNA Polymerase
Taq DNA Polymerase
10X
No
Standalone
2 mL
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For Research Use Only. Not for use in diagnostic procedures.