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Descrizione
Optimize routine PCR with this highly thermostable DNA polymerase.
Thermo Scientific ™ Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.
PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
- Thermostable -half life is more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
- Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Specifica
Specifica
| Content And Storage | 2 x 1.25 ml 10X tampone Taq con (NH4)2SO2, 2 x 1.25 ml 10X tampone Taq con KCl 25 e 2 x 1.25 mLmM MgCl2 |
| Product Type | DNA polimerasi Taq (ricombinante), 1 U/μl |
| Quantità | 100 unità |
| Da utilizzare con (applicazione) | Standard PCR, DNA Sequencing |
| Termostabilità | Emivita superiore a 40 min. a 95 °C |
In certain countries use of this product is covered by patents. Purchase of product in these countries includes non-transferable, limited license for using only this amount of product for the purchaser's own internal research.
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