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Thermo Scientific™ Kit per PCR diretta per tessuti animali Phire™
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Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds, and insects. The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized PCR reagents, dilution buffer, and DNARelease additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes control primers for positive control reactions that are universal and work with numerous animal species.
Features
- No need for time-consuming and expensive DNA purification steps
- Very little sample material required
- Two simple protocols for various applications
- Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb).
Notes
- A detailed manual is provided that describes protocols when working with different types of tissues.
- The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.
Specifica
Specifica
| Content And Storage | • Phire Hot Start II DNA Polymerase, 200 μL • 2X Phire Animal Tissue PCR Buffer, 5 x 1.25 mL • Control Primer Mix (25 μM each), 40 μL • Dilution Buffer, 2 x 5 mL • DNARelease Additive, 3 x 100 μL • Gel Loading Dye, 3 x 1 mL Store at -20°C. |
| GC-Rich PCR Performance | Medium |
| PCR Method | Direct PCR |
| Polimerasi | Phire Hot Start II DNA Polymerase |
| Reaction Speed | Fast |
| Product Type | Direct PCR Kit |
| Quantità | 500 reactions |
| Shipping Condition | Wet or Dry Ice |
| Hot Start | Built-In Hot Start |
| N. di reazioni | 500 reactions |
| Vedi altri risultati |
Sicurezza e movimentazione
- Phire Animal Tissue Direct PCR Kit/DNARelease Additive
- Pericolo
- Sensibilizzante delle vie respiratorie Categoria 1
- H334-Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
- P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P284-¢Quando la ventilazione del locale è insufficiente! indossare un apparecchio di protezione respiratoria.
- P342+P311-In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI/un medico/ .
- P304+P340-IN CASO DI INALAZIONE: trasportare l'infortunato all'aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
- P501-Smaltire il prodotto/recipiente in .
- MIXTURE LIST-Contiene: Proteinase, tritirachium album serine
For Research Use Only. Not for use in diagnostic procedures.
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