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Thermo Scientific™ Polimerasi di DNA di Taq con concentrazione alternativa di MgClsub2sub ThermoPrme™
Descrizione
These Thermo Scientific™ ThermoPrime Taq DNA Polymerases are recommended for PCR applications requiring increased yield and sensitivity and to produce large amounts of PCR product.
High Purity—94kDa ultrapure recombinant thermostable DNA polymerase provides 5« to 3« exonuclease (strand displacement) activity and 3« adenylation activity
High thermal stability—Proprietary buffer formulation protects ThermoPrime Taq during high temperature steps, ensuring optimal performance throughout the PCR reaction
Full-length amplification—ThermoPrime Taq is proven to amplify standard DNA targets in excess of 12kb
Simple protocol—Master mix formats reduce protocol steps and minimize the risk of pipetting errors and sample contamination and are available in ReddyMix format for direct loading onto agarose gels
Reproducible results—ThermoPrime Taq is fully licensed for PCR, and all mixes undergo extensive quality control testing to ensure batch-to-batch consistency
Mix contains: 0.025U/μL ThermoPrime Plus DNA Polymerase (=1.25U/50μL rxn); 75mM Tris-HCl (pH 8.8 at 25°C); 20mM (NH4)2SO4; 0.01% (v/v) Tween™; 200μM of each dATP, dCTP, dGTP, dTTP; 1.5-4.0mM MgCl2 (depending on Cat. No. selected)
Recommended for:
Routine PCR using genomic, viral and plasmid templates; Amplifying fragments up to 12kb; RT-PCR
Specifica
Specifica
| Concentrazione | 2X |
| Content And Storage | DNA polimerasi ThermoPrime Plus 0.025 U/µl (= 1.25 U/50 µl rxn); Tris-HCl 75 mM (pH 8.8 a 25 °C); (NH4)2SO4 20 mM; 0.01% (v/v) Tween™; 200 µM per ciascuno di dATP, dCTP, dGTP, dTTP; MgCl2 1.5-4.0 mM (a seconda del n. di catalogo scelto). Conservare a -20 °C per periodi prolungati. Stabile a 4 °C fino a quattro settimane. Evitare cicli di congelamento/scongelamento. |
| Descrizione | Master Mix; 2 X Concentrazione; 1600 x 25 ul rxn |
| Quantità | 1600 x 25 ul rxn |
| Da utilizzare con (applicazione) | Standard PCR, RT-PCR |
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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