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IBA Lifesciences

IBA Lifesciences provides high performance research tools for cell and protein isolation based on their proprietary Strep-tag™ technology. Their product portfolio offers solutions for the entire production chain of recombinant proteins, from cloning, transfection, protein expression and purification to detection, immobilization and assays, as well as innovative cell isolation and expansion tools.

 

IBA's Cell Selection and Expansion

TACS - Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection:

  • High purity, yield and viability
  • Label-free, non-activated cells due to fully reversible binding
  • From whole blood, buffy coat, PBMCs or murine samples
  • Affinity chromatographic, magnetic or fluorescent cell separation
TACS - Traceless Affinity Cell Selection

MHC I Streptamer™

Detection and isolation of antigen-specific T-cells with MHC I Streptamers™:

  • Label-free cell isolation according to antigen specificity
  • High specificity, sensitivity, and reproducibility
  • From single cell suspensions (e.g., PBMCs or murine samples)
  • Affinity chromatographic, magnetic or fluorescent cell separation
MHC I Streptamer™

Cell Stimulation and Expansion

CD3: CD28 Streptamers™:

  • Completely reversible reagents
  • Termination of stimulation at any given point in time
  • Adjustable CD3: CD28 ratio 
  • Bead-free

Peptides for T-Cell Stimulation:

  • Purity >85%
  • Endotoxin-free
Cell Stimulation & Expansion

Fluorescent Cell Staining

Detection of antigen-specific T-cells with MHC I Streptamers™ and of receptor-specific T-cells with Fab Streptamers™:

  • Fully reversible
  • High specificity, sensitivity, and reproducibility
  • No leftover staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Fluorescent Cell Staining

Protein Production and Assay

Cloning

More than 150 Twin-Strep-tag™/Strep-tag™II expression vectors:

  • Variety of Twin-Strep-tag™/Strep-tag™ II combined with His, FLAG or GST
  • Different N-/C-terminal orientations
  • Expression hosts: E. coli, mammalia, insect cells, yeast
  • Easy cloning via StarGate vectors
Cloning

Mammalian Expression

The MEXi mammalian expression system is optimised for efficient protein expression and Strep-tag™ purification in mammalian cells:

  • Perfectly matched HEK293E cell line and cell culture media
  • Transient expression for time and cost efficient protein production
  • Multiple expression vectors with different features
Mammalian Expression

Protein Purification

Strep-tag™ technology from the original manufacturer:

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (>95%)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, and protein complexes amongst others
Protein Purification

Detection

For Western blot analysis, immunofluorescence, FACS and ELISA.

Two different detection systems are provided:

  • Strep-Tactin™ and Strep-Tactin™ XT conjugate for Twin-Strep-tag™/Strep-tag™ II proteins
  • Monoclonal antibodies against Twin-Strep-tag™/Strep-tag™ II
Detection

Immobilization

With near covalent binding affinity, – Immobilisation by Strep-Tactin XT: Twin-Strep-tag can be used for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, surface plasmon resonance (SPR) experiments (Biacore) or bio-layer interferometry (BLI):

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated
Immobilisation

Surface-Marker Specific Exosome Isolation

Quick and simple isolation of unlabeled exosomes using Fab-TACS™ for exosomes, which offers:

  • Highly specific and reproducible isolation
  • Minimal contamination by other EVs
  • Label-free exosomes with intact biological functions
  • Source-independent application
  • Reversible capture and release of exosomes
  • A quick and simple protocol, without magnetic beads or ultracentrifugation
Surface-marker Specific Exosome Isolation

Immobilization with Near Covalent Binding Affinity

Reversible capture and analysis of Twin-Strep-tag™ fusion proteins for bio-layer interferometry (BLI) or surface plasmon resonance (SPR) experiments:

  • Indirect immobilization of the ligand prevents it from being subject to inactivation or influences of the immobilization procedure
  • High specificity and affinity in low pM range of the Twin-Strep-tag™ - Strep-Tactin™ XT interaction
  • Ligands can be captured directly from complex samples
  • Strep-Tactin™ XT biosensors/chips can be easily regenerated by an established procedure, whereby further ligand-analyte interactions can be analyzed with the same biosensor/chip
Immobilization with Near Covalent Binding Affinity

Our Perfect Tool for Isolation of Proteins or Complexes

MagStrep 'Type 3' XT Magnetic Beads offer:

  • High specificity and low background resulting in high protein purity
  • High binding affinity and capacity for Strep-tag™ II or Twin-Strep-tag™ fusion proteins
  • Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) in batch format and/or high throughput screening for protein purification via pull-downs
  • Suitable for usage with (semi-)automated magnetic bead purification systems (e.g., Thermo Scientific™ KingFisher™) for automated workflows
Our Perfect Tool for Isolation of Proteins or Complexes

Efficient Purification Even of Large Proteins

The Strep-Tactin™ XT 4Flow™ Starter Kit consists of everything you need for a quick start:

  • Strep-Tactin™ XT 4Flow™ gravity flow column
  • All buffers required for washing, elution, and regeneration
  • Strep-Tactin™ conjugated with horseradish peroxidase (HRP; for Western blot detection)
  • Short protocol
Efficient Purification Even of Large Proteins

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

Applicable for purification, detection, and immobilization of biotinylated molecules:

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

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1 – 15 of 508 results
1

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity 50% suspension

Strep-Tactin™ 4Flow™ high capacity 50% Suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Particle Size 50 to 150 μm
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Type Bulk Resin
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Format Bottle
Description 4% agarose, crosslinked
Final Product Type Affinity chromatography equipment
Protein Form Tetrameric protein
Form Resin Slurry
Binding Capacity 20 mg/mL resin
Column Type Suspension
Concentration 50% suspension
2

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Resin
BIOPHARMA

Strep-Tactin™XT 4Flow™ high capacity resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

3

IBA Lifesciences Strep-Tactin™XT 4Flow™ Resin
BIOPHARMA

Strep-Tactin™XT 4Flow™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

4

IBA Lifesciences StrepMAB-Classic Conjugate
GREENER_CHOICE

StrepMAB-Classic conjugates are monoclonal antibodies with different conjugates to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins

Type Conjugate
Target Strep-tag™II and Twin-Strep-tag™
Content And Storage Undiluted: 2°C to 8°C, protect from light; Diluted: -25°C to -15°C
5

IBA Lifesciences Strep-Tactin™ Conjugate

Strep-Tactin™ conjugates with AP or HRP for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins

6

IBA Lifesciences 10x Buffer R - Regeneration Buffer

10x Buffer R is a Strep-Tactin™ regeneration buffer with HABA

7

IBA Lifesciences Strep-Tactin™ protein as lyophilized powder
GREENER_CHOICE

Strep-Tactin™ protein as lyophilized powder can be used for reversible immobilization of biotinylated analytes or Strep-tag™II and Twin-Strep-tag™ fusion proteins

Binding Capacity >50 nmol/mg
8

IBA Lifesciences Gravity flow Strep-Tactin™ 4Flow™ high capacity column

Strep-Tactin™ 4Flow™ high capacity gravity flow column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Particle Size 50 to 150 μm
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Format Gravity flow column
Description 4% agarose, crosslinked
Final Product Type Affinity chromatography equipment
Protein Form Tetrameric protein
Form Resin Slurry
Binding Capacity 20 mg/mL resin
Column Type Pre-packed gravity flow column
9

IBA Lifesciences Gravity flow Strep-Tactin™ 4Flow™ high capacity mini- column

Strep-Tactin™ 4Flow™ high capacity gravity flow mini- column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

10

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity FPLC column

Strep-Tactin™ 4Flow™ high capacity FPLC column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Particle Size 50 to 150 μm
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Type Column
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Description 4% agarose, crosslinked
Final Product Type Affinity chromatography equipment
Protein Form Tetrameric protein
Form Resin Slurry
Binding Capacity 20 mg/mL resin
Quantity 5 mL
11

IBA Lifesciences 10x Buffer CI

Cell isolation buffer containing 10x PBS with 10 mM EDTA and 5% BSA

12

IBA Lifesciences Streptavidin
BIOPHARMA GREENER_CHOICE

Streptavidin is a widely used tool for immobilization and assays of biotinylated proteins or other biotin conjugated molecules.

Particle Size 13.3 kDa/subunit
Purity or Quality Grade >95 %
Source Recombinant, expression in E. coli
13

IBA Lifesciences Strep-Tactin™XT 4Flow™ Starter Kit

Strep-Tactin™XT 4Flow™ Starter Kit contains all reagents required for purification and detection of Strep-tagII™ and Twin-Strep-tag™ fusion proteins

14

IBA Lifesciences Strep-Tactin™XT Conjugate
GREENER_CHOICE

Strep-Tactin™XT conjugates with fluorescent label to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins

Type Conjugate
Target Strep-tag™II and Twin-Strep-tag™
Content And Storage Undiluted: 2°C to 8°C, protect from light; Diluted: -25°C to -15°C
Kit Contents Solution in PBS; 0.01 % NaN3; 1 mg/ mL BSA
Capacity (Metric) 0.10 mL
Quantity 50 μg
Concentration 0.5 mg/mL
15

IBA Lifesciences StrepMAB-Immo Conjugate
GREENER_CHOICE

StrepMAB-Immo conjugates are monoclonal antibodies with different fluorescent conjugates to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins

Type Conjugate
Target Strep-tag™II and Twin-Strep-tag™
Content And Storage Undiluted: 2°C to 8°C, protect from light; Diluted: -25°C to -15°C
Kit Contents Solution in PBS; 0.01 % NaN3; 1 mg/ mL BSA
Capacity (Metric) 0.10 mL
Quantity 50 μg
Concentration 0.5 mg/mL
For Use With (Application) ELISA, FACS, immunofluorescence

Cell Selection and Expansion

Application Note Central memory T-cells isolated and stained with CD8α and CD62L Fab Streptamer reagents
Filename
ApplicationNote-Central-memory-T-cells-isolated-and-stained-with-CD8α-and-CD62L-Fab-Streptamer-reagents.pdf
Size
662 KB
Format
application/pdf
Application Note-Combining-MHC-I-and-CD8+-Fab-Streptamer-reagents
Filename
Application_Note_Combining.pdf
Size
803 KB
Format
application/pdf
Application Note Regulatory T-cells isolated and stained with CD4-CD25 and CD45RA Fab Streptamer reagents
Filename
ApplicationNote-Regulatory-T-cells-isolated-and-stained-with-CD4-CD25-and-CD45RA-Fab-Streptamer-reagents.pdf
Size
737 KB
Format
application/pdf
Application Note CD3-T Lymphocytes isolated and stained with Streptamer reagents
Filename
ApplicationNote-CD3-T-Lymphocytes-isolated-and-stained-with-Streptamer-reagents.pdf
Size
200 KB
Format
application/pdf
Application Note Negative Cell Selection Using Affinity Chromatography
Filename
Application_Note_Negative_cell_selection_using_affinity_chromatography.pdf
Size
1 MB
Format
application/pdf
Application Note Depletion Of Cell Populations From Different Samples
Filename
Application_Note_Depletion_of_cell_populations_from_different_samples.pdf
Size
1 MB
Format
application/pdf
White Paper Understanding The World Of Mhc I Multimers
Filename
White_Paper_Understanding_The_World_of_MHC_I_Multimers.pdf
Size
5 MB
Format
application/pdf

Protein Production and Assay

ApplicationNote-Strep-Tactin-XT-vs-Strep-Tactin-high-capacity

ApplicationNote-Strep-Tactin-XT-vs-Strep-Tactin-High-Capacity

Filename
ApplicationNote-Strep-Tactin-XT-vs-Strep-Tactin-high-capacity.pdf
Size
193 KB
Format
application/pdf
Application Note Strep Tactin high capacity
Filename
ApplicationNote-Strep-Tactin-high-capacity.pdf
Size
100 KB
Format
application/pdf
Application Note Strep Tactin XT Assay
Filename
ApplicationNote-Strep-Tactin-XT-Assay.pdf
Size
261 KB
Format
application/pdf
Application Note Strep Tactin XT vs Strep Tactin high capacity
Filename
ApplicationNote-Strep-Tactin-vs-Strep-Tactin-XT.pdf
Size
917 KB
Format
application/pdf
Application Note Twin Strep tag vs His tag
Filename
ApplicationNote-Twin-Strep-tag-vs-His-tag.pdf
Size
675 KB
Format
application/pdf
Application Note The Strep-Tactin Xt Technology In Downstream Applications
Filename
Application_note_BLISPR_with_Strep-TactinXT.pdf
Size
1 MB
Format
application/pdf
White Paper Comprehensive Comparison Of The Strep-Tag Technology With The His-Tag System
Filename
White_paper_Comparison_protein_purification_systems.pdf
Size
1 MB
Format
application/pdf
White Paper Transient Expression In Mammalian Cells
Filename
White_paper_Transient_expression_in_mammalian_cells.pdf
Size
1 MB
Format
application/pdf

Exosomes

Isolation of Exosomes from Mesenchymal Stem Cells
Filename
Application_note_exosome_isolation_from_MSC.pdf
Size
1 MB
Format
application/pdf
Isolation of Exosomes from Jurkat Cells and Human Serum
Filename
Application_note_exosome_isolation_from_Jurkat_Serum.pdf
Size
782 KB
Format
application/pdf