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LAMP and Other Isothermal Amplification Technologies

Nucleic acid (RNA and DNA) amplification products designed to amplify targeted material at a constant temperature as opposed to thermocylcing. Products include loop-mediated isothermal amplification (LAMP) assays as well as other isothermal amplification reagents, kits, and buffers. Products are well suited for detecting a variety of pathogens from a broad array of source material, including animal, plant, and environmental samples.
Isothermal Amplification Reagents (9)
LAMP Assays (2)

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Thermo Scientific™ EquiPhi29™ DNA Amplification Kit

The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).

EDGE
Tempo di reazione Up to 2 hours
Shipping Condition Dry Ice
Polymerase EquiPhi29 DNA Polymerase
Forma Liquid
Product Type DNA Amplification Kit
Da utilizzare con (applicazione) RCA,MDA-WGA
Research Category Nucleic Acid Analysis,In Vitro Transcription,Cell-free Protein Expression,In Situ Sequencing,SNP Detection
Optimal Reaction Temperature 42°C
Formato Kit
2
Promozioni disponibili

Thermo Scientific™ EquiPhi29™ DNA Polymerase

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.

EDGE
Tempo di reazione 2 hours
Shipping Condition Dry Ice
Polymerase EquiPhi29 DNA Polymerase
Product Type Stand-alone enzyme
Da utilizzare con (applicazione) MDA-WGA,RCA
Optimal Reaction Temperature 42°C
Concentrazione 10 U/μL
3
Promozioni disponibili

Thermo Scientific™ DNA polimerasi phi29

Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.

EDGE
Tempo di reazione 4 hours
Shipping Condition Dry Ice
Polymerase phi29 DNA Polymerase
Product Type Stand-alone enzyme
Da utilizzare con (applicazione) MDA-WGA,RCA
Optimal Reaction Temperature 30°C
Concentrazione 10 U/μL
4
Promozioni disponibili

Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.

EDGE
5

Invitrogen™ Colorimetric ReadiLAMP™ Kit, SARS-CoV-2

The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.

Da utilizzare con (apparecchiatura) Heating block
Enzimi SuperScript IV & proprietary Bst-derived DNA polymerase
Colore During reaction color changes from purple to blue
Metodo di rilevamento Colorimetric readout
Limite di rilevamento <250 copies/25 μL (Direct LAMP)
<100 copies/25 μL (Purified RNA-based LAMP)
Tasti funzione Detects SARS-CoV-2 presence
Da utilizzare con (applicazione) SARS-CoV-2 surveilance
Tipo di profilo Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads
Include LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA
Tipo di campione Saliva or Nasopharyngeal/Nasal Swab
Formulazione Liquid. 5 components included in the kit.
Volume del campione Range of sample inputs
6
Promozioni disponibili

Thermo Scientific™ Reaction Buffer for phi29 DNA Polymerase (10X)

Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi29 DNA polymerase.