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Thermo Scientific™ DNA polimerasi Taq, ricombinante
Optimize routine PCR with this highly thermostable DNA polymerase.
Marca: Thermo Scientific™ EP0405
915.44 EUR valido fino al 2024-05-31
Usa il codice promo "21664" per avere il tuo prezzo promozionale.
Dettagli aggiuntivi : Peso : 2.44490kg
Descrizione
Optimize routine PCR with this highly thermostable DNA polymerase.
Thermo Scientific ™ Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.
PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
- Thermostable -half life is more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
- Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Specifica
| Contains: • 5 x 100 μL Taq DNA Polymerase (5 U/μL) • 10 x 1.25 mL 10X Taq Buffer with KCl • 10 x 1.25 mL 10X Taq Buffer with (NH4)2SO4 • 10 x 1.25 mL 25 mM MgCl2 Store at -20°C. |
|
| Taq DNA Polymerase | |
| Taq DNA Polymerase (recombinant) (5 U/μL) | |
| 1 X | |
| 3'-A |
| Low | |
| Standard | |
| 5 x 500 Units | |
| No | |
| Standalone |
For Research Use Only. Not for use in diagnostic procedures.