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Cytiva Colonne preimpaccate HiTrap™ TALON™ grezzo
Per la purificazione di proteine ricombinanti con tag di istidina tramite cromatografia di affinità con ioni metallici immobilizzati (IMAC). Le colonne preimpaccate grezze Cytiva Lifescience™ HiTrap™ TALON™ offrono un terreno a base di cobalto che consente la purificazione delle proteine in condizioni native o denaturanti.
Marca: Cytiva 29048565
Dettagli aggiuntivi : CAS : 67-56-1 Peso : 0.10200kg
Descrizione
Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
- Prepacked with cobalt-based chromatography media
- Binding capacity approximately 7mg MBP
- Maximum pressure: 3 bar
- Column material: polypropylene
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
- 1 and 5mL columns
Media
- Ligand: TALON™ Superflow Cobalt-IMAC
- Matrix: 6% cross-linked agarose
- Average particle size: 60 to 160μm
- pH stability working range: 3 to 12
1mL columns
- Column I.D.: 7mm
- Flow velocity: 1 to 4mL/min.
5mL columns
- Column I.D.: 16
- Flow velocity: 5 to 20mL/min.
Specifica
| 1 x 1 mL | |
| 4°C to 8°C | |
| 0.27 in. | |
| 0.7 cm | |
| 60 to 160 μm | |
| 3 bar | |
| 3 to 12 | |
| Talon Superflow Cobalt-IMAC |
| For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH | |
| Up to 20 mg Histidine-tagged Protein1 | |
| HiTrap Talon crude prepacked Column | |
| 1 to 4 mL/min | |
| Polypropylene | |
| 6% Cross-Linked Agarose | |
| 20% Ethanol | |
| 1 x 1 mL |
Sicurezza e movimentazione
Avvertenza
Categoria di pericolo
- Attenzione
Categoria di pericolo
- Liquido infiammabile Categoria 3