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Cytiva Kit di amplificazione DNA illustra™ a cella singola GenomiPhi™
Per amplificare il DNA di un intero genoma da un'unità piccola come una singola cellula. Il kit di amplificazione del DNA Cytiva Lifescience™ illustra™ Single Cell GenomiPhi™ combina le esclusive capacità della DNA polimerasi Phi29 con una formulazione ottimizzata per amplificare il DNA genomico da 1 a 1000 cellule senza interferenze di background.
Marca: Cytiva 29108039
Dettagli aggiuntivi : Peso : 0.00500kg Transport : UN number : 1814 Chem class : 8 Pack group : II
Descrizione
- For whole genome amplification of genomic DNA from as little as a single cell
- Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
- Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
- Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
- Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
- Reaction time: 2 to 4 hours
- Product average size: >10kb
For SNP arrays, Next Gen Sequencing, aCGH
Recommended StorageStore the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C
Specifica
| Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. | |
| illustra™ Single Cell GenomiPhi™ DNA Amplification Kit | |
| Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL | |
| illustra Single Cell GenomiPhi DNA Amplification Kit | |
| 2 to 4 hours |
| Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference. | |
| Genomic DNA Amplification | |
| 100 | |
| >10kb |
Sicurezza e movimentazione
Avvertenza
Categoria di pericolo
- Pericolo
Categoria di pericolo
- Corrosione/irritazione della pelle Categoria 1