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Thermo Scientific™ Miscela master per PCR Phusion™ Flash High-Fidelity
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.
Marca: Thermo Scientific™ F548S
87.41 EUR valido fino al 2024-04-30
Usa il codice promo "21741" per avere il tuo prezzo promozionale.
Dettagli aggiuntivi : Peso : 1.51950kg
Descrizione
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in the reaction when using extremely short PCR protocols. Additionally, the user only needs to add template and primers minimizing the number of pipetting steps.
The unique master mix composition permits usage of extremely short cycling protocols with both low and high complexity DNA templates - 15 seconds per kilobase or less. The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.
Features
• Extreme speed: extension times of 15 s/kb or less
• Hot start modification allowing 'zero-time reactivation'
• Accuracy: proofreading DNA polymerase with a fidelity of 25X Taq
• High yields in reduced time
Applications
• Fast PCR
• High-fidelity PCR
• Difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning
• Mutagenesis
• Microarray
Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.
Specifica
| Phusion Flash High-Fidelity PCR Master Mix (1 mL) Store at -20°C. |
|
| Phusion Flash II DNA Polymerase | |
| High-Fidelity PCR Master Mix | |
| Hot-start PCR, High-fidelity PCR | |
| 25X | |
| 100 Reactions | |
| SuperMix or Master Mix |
| High | |
| Fast | |
| Dry Ice | |
| 2X | |
| Built-In Hot Start | |
| Blunt | |
| 20 kb or less |
For Research Use Only. Not for use in diagnostic procedures.