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Thermo Scientific™ Kit mutagenesi diretta al sito Phusion

Generate mutations with this fast and reliable system.

Marca:  Thermo Scientific™ F541

Dettagli aggiuntivi : Peso : 1.71460kg

Codice prodotto. 10493438

  • 236.00€ / 20 reazioni
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Descrizione

Descrizione

Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into any competent E. coli cells.

Highlights

  • Robust and reliable exponential amplification method
  • No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid
  • No need to destroy the starting template in a separate step
  • Phusion Hot Start II High Fidelity DNA Polymerase minimizes unwanted secondary mutations
  • Amplification of large plasmids up to 10 kb
  • Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR
  • T4 DNA Ligase included in the kit; no purification steps before or after ligation
  • Compatible with all strains of competent E. coli cells
Specifica

Specifica

Solution
High
Fast
Phusion
52X
20 Reactions
Kit
Phusion Hot Start II High Fidelity DNA Polymerase
Single-Site Mutagenesis
Site-Directed Mutagenesis Kit
Yes
DNA
Suggerimenti di prodotto

Suggerimenti di prodotto

Videos
SDS
Documenti

Documenti

Certificati
Offerte speciali

Offerte speciali

For Research Use Only. Not for use in diagnostic procedures.