Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Corning™ Cell Culture Phosphate Buffered Saline (1X)
With low endotoxin content. Without calcium and magnesium.
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Tamponi di uso comune
Corning™ Soluzione tampone salino fosfato
Used alone or in combination with other agents for washing tissues and cells.
PBS, 10X Solution, Molecular Biology Grade
For biological research. Affymetrix/USB™ PBS, 10X Solution, Molecular Biology Grade is 0.2μm filtered and dispensed into durable, square nalgene bottles and upright carton.
Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4
Non-toxic to most cells and is used in biological research.
Compresse PBS, tampone fosfato salino, Fisher BioReagents
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
Corning™ Cell Culture Buffers: Dulbecco's Phosphate-Buffered Salt Solution 1X
With low endotoxin content.
MP Biomedicals™ Phosphate Buffered Saline Tablets (PBS)
Phosphate Buffered Saline Tablets (PBS) are a balanced salt mixture for easy PBS buffer preparation for a variety of applications, including reagent preparation, diluting cells for flow cytometry, and cell culture reagent.
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Formula molecolare: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Sinonimo: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
MOPS (Fine White Crystals/Molecular Biology), Fisher BioReagents™
CAS: 1132-61-2 Formula molecolare: C7H15NO4S Molecular Weight (g/mol): 209.26 Numero MDL: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Sinonimo: 3-(4-Morpholino)propane sulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: acido 3-morfolin-4-il-propano-1-solfonico SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
Tris(Hydroxymethyl) Methylamine, 'Tris Buffer', 99+%, Extra Pure, SLR, Fisher Chemical™
CAS: 77-86-1 Formula molecolare: C4H11NO3 Molecular Weight (g/mol): 121.136 Numero MDL: 4679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™
Agente tamponante molto diffuso
Thermo Scientific Chemicals Krebs-Ringer Solution, HEPES-buffered
Standard physiological buffer that is suitable for various calcium imaging experiments. It is also used as a preserving agent.