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Thermo Scientific™ Kit per la purificazione di DNA genomico
Isolate genomic DNA from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues, with this DNA precipitation system.
Thermo Scientific™ Kit GeneJET™ Plasmid Miniprep
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.
Compatibilità ad alta produttività | Compatible (Vacuum manifold) |
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Product Type | Plasmid MiniPrep Kit |
Tipo di colonna | Spin Column |
Isolation Technology | Silica Column |
Da utilizzare con (applicazione) | PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription |
Bilancia | Mini |
Linea di prodotti | GeneJET |
Durata del test | <14 min |
Tipo di campione | Bacteria |
Bersaglio | Plasmid DNA |
Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
Formato | Spin Column |
Thermo Scientific™ DNA polimerasi Phusion High-Fidelity
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion HF & GC Buffers • DMSO • 50 mM MgCl2 solution Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration. Store at -20°C. |
Reaction Format | Standalone |
Product Type | High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | No |
Da utilizzare con (applicazione) | Standard PCR,High-fidelity PCR |
Sporgenza | Blunt |
Concentrazione | 2 U/μL |
Dimensioni (prodotto finale) | 20 kb or less |
Fedeltà (rispetto a taq) | 52X |
Thermo Scientific™ ATP
Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Thermo Scientific™ Esente da RNasi A, DNasi e proteasi
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
Thermo Scientific™ dNTP Mix (10 mM each)
Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.
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Shipping Condition | Dry Ice |
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Purity or Quality Grade | >99% |
Forma | Liquid |
Label or Dye | Unlabeled |
Concentrazione | 10 mM |
Thermo Scientific™ Inibitore di RNasi RiboLock
RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.
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Product Type | RNase Inhibitor |
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Compatible Buffer | Storage Buffer |
Concentrazione | 40 U/μL |
Product Line | RiboLock™ |
Thermo Scientific™ Proteinasi K (ricombinante), PCR
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Forma | Liquid |
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Product Type | Proteinase K |
Thermo Scientific™ Tampone TAE 50X (Tris-acetato-EDTA)
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).
Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)
Save time in PCR applications with Master Mix and get higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
GC-Rich PCR Performance | Low |
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Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Polimerasi | DreamTaq DNA Polymerase |
Linea di prodotti | DreamTaq™ |
Sporgenza | 3'-A |
Fedeltà (rispetto a taq) | 1 X |
Thermo Scientific™ Esente da DNasi I, RNasi
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ Albumina da siero bovino (BSA)
Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.
Thermo Scientific™ T4 DNA ligasi
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
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Product Type | T4 DNA Ligase |
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Enzyme | DNA Ligase |
Compatible Buffer | 10X T4 DNA Ligase Buffer |
Concentrazione | 5 U/μL |
Thermo Scientific™ FastDigest™ NotI
The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).
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Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Not I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ Kit di clonazione per PCR CloneJET™
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
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Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
Da utilizzare con (applicazione) | PCR Cloning |
Vettore | pJET1.2/blunt |
Linea di prodotti | CloneJET |
Tipo di campione | PCR Products |
Formato | Kit |