Desalting Columns
Disposable or reusable gel filtration chromatography columns for use in protein biology or DNA extraction and purification, for the fractional purification of proteins, nucleic acids, and other macromolecules from low molecular-weight buffer salts and reagents.
Desalting columns are special kinds of gel filtration (exclusion) chromatography columns suitable for the fractional purification of proteins, nucleic acids, and other macromolecules. Their use offers a more convenient and quicker alternative to dialysis.
Desalting columns may be disposable or reusable plastic or glass columns prepacked with beads of a suitable media, typically polysaccharide (Sephadex), or porous polyacrylamide that are more resistant to enzymatic degradation. The beads have defined molecular weight cut-off (MWCO) exclusion points (typically around 6000 Da) that trap, retain, and slow down the elution of small molecules below the MWCO, but exclude and therefore do not impede the more rapid elution of larger macromolecules such as proteins.
Desalting columns can achieve high sample recovery yields of >95%, with column capacities ranging from 5 μL to 10 mL that are suitable for applied sample volumes anywhere between 2 μL and 4 mL.
Types of Desalting Columns
• Columns designed for simple gravity flow
• Spin columns designed especially for centrifugation
• Pressure-compatible columns designed for use with suitable pumps or with integrated preparative LC systems
How Are Desalting Columns Used?
• Removal of salts from protein samples
• Buffer exchange prior to downstream electrophoresis or affinity chromatography
• Removal of unreacted radioactive labels
• Removing phenol or free nucleotides from nucleic acid preparations
• Removing of primers or dyes from PCR or sequencing reactions
• Removal of other low molecular weight compounds such as cross-linking, labelling or derivatization reagents, cofactors, inhibitors, or other contaminants
Thermo Scientific™ Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL
The new and enhanced Zeba Spin and Micro Spin Desalting columns and plates are designed for efficient desalting and buffer exchanging proteins with a molecular weight >40 kDa and removing small molecules less than 2 kDa. They provide exceptional desalting and protein recovery performance.
Cytiva Colonne per desalinizzazione monouso PD-10
Adatte per desalinizzazione, scambio di tamponi e rimozione di composti a basso peso molecolare. Le colonne di desalinizzazione Cytiva Life Sciences™ PD-10 monouso garantiscono un recupero elevato e rapido.
Thermo Scientific™ Colonne per desalinizzazione in poliacrilamide Pierce™
Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with Thermo Scientific Pierce 7K MWCO (molecular-weight cutoff) Polyacrylamide Spin Desalting Columns, which are porous polyacrylamide-based gel-filtration columns for biological sample preparation.
Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
Thermo Scientific™ Pierce™ Polyacrylamide Desalting Columns, 1.8K MWCO, 5 mL
Thermo Scientific Pierce 1.8K MWCO (molecular-weight cutoff) Polyacrylamide Desalting Columns are ready-to-use, disposable, gel-filtration columns for separating proteins and other macromolecules from low-molecular-weight buffer salts and reagents.
Cytiva Colonne per desalinizzazione PD SpinTrap™ G-25
Per eseguire in modo pratico e veloce una pulizia su piccola scala di proteine e altre biomolecole grandi (>5000 MR). Le colonne per desalinizzazione Cytiva Life Sciences™ PD SpinTrap™ G-25 offrono la possibilità di operare su più campioni in contemporanea.
Cytiva Colonne di desalinizzazione preimpaccate HiTrap™
Per desalinizzazione e scambio dei tamponi in modo rapido e pratico. Le colonne preimpaccate HiTrap™ desalinizzanti sono pronte per l'uso per la separazione tra gruppi di sostanze con peso molecolare alto e basso.
Cytiva Colonne di desalinizzazione HiPrep™ 26/10
Affidabilità e riproducibilità per desalinizzazione e scambio dei tamponi con campioni fino a 15 ml per colonna. La colonna di desalinizzazione Cytiva HiPrep 26/10 è composta da una pratica colonna da 53 ml di facile uso preimpaccata con Sephadex G-25 Fine per desalinizzazione e scambio dei tamponi.
Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL
Enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.
Thermo Scientific™ Pierce™ Dextran Desalting Columns (5K MWCO)
Ready-to-use, gel-filtration Thermo Scientific Pierce Dextran Desalting Columns can separate proteins and macromolecules from low-molecular weight buffer salts and reagents up to 5000 Daltons (5 kDa) in 5- or 10-mL volumes.
Cytiva Colonne PD-10 monouso
Per purificazione con flusso di gravità. Colonne PD-10 monouso: Le colonne vuote in confezioni da 50 sono economiche, in plastica, e possono essere confezionate con un'ampia gamma di terreni disponibili in stock.
Cytiva™ MiniTrap™ Column
The Cytiva™ MiniTrap™ Columns are small-scale chromatography columns designed for rapid and convenient purification. They are commonly used for various applications including protein purification, antibody purification, peptide purification and nucleic acid purification.
Cytiva Colonna preimpaccata Superdex 75 Increase, 10/300 GL
Colonne per la purificazione preparativa e per la caratterizzazione e l'analisi di proteine con massa molecolare relativa compresa tra 3.000 e 70.000 Mr, quali proteine marcate ricombinanti. Le colonne preimpaccate Cytiva HisTrap™ excel tollerano bene trattamenti di pulizia ripetuti con soluzioni corrosive a pH elevato garantendo una lunga durata delle colonne e una ridotta contaminazione da residui.
Cytiva Superdex 75 Increase Prepacked Column, 5/150 GL
Use this column for rapid purity check and screening of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva Superdex 75 Increase Prepacked Columns are ideal when time is limited and low buffer and sample consumption is important.
Cytiva Superdex 75 Increase Prepacked Column, 3.2/300
Use this column for rapid purity check and screening of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva Superdex 75 Increase Prepacked Columns are ideal when time is limited and low buffer and sample consumption is important.
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